Anti-Nuf2 Rabbit Monoclonal Antibody

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF, ICC, FC
Primary Accession	Q9BZD4
Host	Rabbit
Isotype	IgG
Reactivity	Human
Clonality	Monoclonal
Format	Liquid
Description	Anti-Nuf2 Rabbit Monoclonal Antibody . Tested in WB, ICC/IF, Flow Cytometry applications. This antibody reacts with Human.

Additional Information
Gene ID	83540
Other Names	Kinetochore protein Nuf2, hNuf2, hNuf2R, hsNuf2, Cell division cycle-associated protein 1, NUF2, CDCA1, NUF2R
Calculated MW	54304 Da
Application Details	WB 1:500-1:2000 ICC/IF 1:100-1:500 FC 1:50
Source	Eukaryota
Contents	Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clone Names	Clone: 18N81
Immunogen	A synthesized peptide derived from human Nuf2
Purification	Affinity-chromatography
Storage	Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	NUF2
Synonyms	CDCA1, NUF2R
Function	Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity (PubMed:12438418, PubMed:14654001, PubMed:15062103, PubMed:15235793, PubMed:15239953, PubMed:15548592, PubMed:17535814). Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore (PubMed:15548592). The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules (PubMed:23085020).
Cellular Location	Nucleus. Chromosome, centromere, kinetochore. Note=Localizes to kinetochores from late prophase to anaphase Localizes specifically to the outer plate of the kinetochore. NDC80 is required for efficient kinetochore localization