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>   首页   >   产品   >   一抗   >   信号转导   >   Anti-FAAH1 Rabbit Monoclonal Antibody   

Anti-FAAH1 Rabbit Monoclonal Antibody

     
  • 1 - Anti-FAAH1 Rabbit Monoclonal Antibody ABO15457
    Figure 1. Western blot analysis of FAAH using anti-FAAH antibody (M00801-1).
    Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
    Lane 1: human SH-SY5Y whole cell lysates,
    Lane 2: mouse brain tissue lysates,
    Lane 3: mouse pancreas tissue lysates.
    After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FAAH antigen affinity purified monoclonal antibody (Catalog # M00801-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FAAH at approximately 63 kDa. The expected band size for FAAH is at 63 kDa.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC
Primary Accession O00519
Host Rabbit
Isotype IgG
Reactivity Human, Mouse
Clonality Monoclonal
Format Liquid
Description Anti-FAAH1 Rabbit Monoclonal Antibody . Tested in WB, IHC applications. This antibody reacts with Human, Mouse.
Additional Information
Other Names Fatty-acid amide hydrolase 1, 3.5.1.99, Anandamide amidohydrolase 1, Fatty acid ester hydrolase, 3.1.1.-, Oleamide hydrolase 1, FAAH, FAAH1
Calculated MW 63 KDa
Application Details WB 1:500-1:2000
IHC 1:50-1:200
Contents Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clone Names Clone: 20F33
Immunogen A synthesized peptide derived from human FAAH1
Purification Affinity-chromatography
Storage Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Research Areas
Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol
Blocking Peptides Protocol
Dot Blot Protocol
Immunohistochemistry Protocol
Immunofluorescence Protocol
Immunoprecipitation Protocol
Flow Cytomety Protocol
Cell Culture Protocol
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¥ 3,367.00
Cat# ABO15457
Size:
100 µl
Price:
¥ 3,367.00
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ABO15457: Anti-FAAH1 Rabbit Monoclonal Antibody
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