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Anti-CEND1 Rabbit Monoclonal Antibody

     
  • 1 - Anti-CEND1 Rabbit Monoclonal Antibody ABO15604
    Figure 1. Western blot analysis of CEND1 using anti-CEND1 antibody (M13043).
    Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
    Lane 1: human U-87MG whole cell lysates,
    Lane 2: human SH-SY5Y whole cell lysates,
    Lane 3: human U251 whole cell lysates,
    Lane 4: human PC-3 whole cell lysates,
    Lane 5: rat brain tissue lysates,
    Lane 6: rat C6 whole cell lysates,
    Lane 7: mouse brain tissue lysates,
    Lane 8: mouse Neuro-2a whole cell lysates.
    After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CEND1 antigen affinity purified monoclonal antibody (Catalog # M13043) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CEND1 at approximately 22 kDa. The expected band size for CEND1 is at 15 kDa.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC, IF, ICC, IP
Primary Accession Q8N111
Host Rabbit
Isotype IgG
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Format Liquid
Description Anti-CEND1 Rabbit Monoclonal Antibody . Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.
Additional Information
Gene ID 51286
Other Names Cell cycle exit and neuronal differentiation protein 1, BM88 antigen, CEND1, BM88
Calculated MW 14954 Da
Application Details WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
Source Eukaryota
Contents Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clone Names Clone: 21C80
Immunogen A synthesized peptide derived from human CEND1
Purification Affinity-chromatography
Storage Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name CEND1
Synonyms BM88
Function Involved in neuronal differentiation.
Cellular Location Membrane; Single-pass type IV membrane protein
Tissue Location Neuron specific..
Research Areas
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