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>   首页   >   产品   >   一抗   >   信号转导   >   Anti-TCP1 beta Rabbit Monoclonal Antibody   

Anti-TCP1 beta Rabbit Monoclonal Antibody

     
  • 1 - Anti-TCP1 beta Rabbit Monoclonal Antibody ABO15640
    Figure 1. Western blot analysis of TCP1 Beta using anti-TCP1 Beta antibody (M05524-2).
    Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
    Lane 1: human Hela whole cell lysates,
    Lane 2: human Raji whole cell lysates,
    Lane 3: human T-47D whole cell lysates,
    Lane 4: human SW620 whole cell lysates,
    Lane 5: rat brain tissue lysates,
    Lane 6: rat PC-12 whole cell lysates,
    Lane 7: mouse brain tissue lysates,
    Lane 8: mouse RAW264.7 whole cell lysates.
    After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TCP1 Beta antigen affinity purified monoclonal antibody (Catalog # M05524-2) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TCP1 Beta at approximately 57 kDa. The expected band size for TCP1 Beta is at 57 kDa.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC, IF, ICC, IP, FC
Primary Accession P78371
Host Rabbit
Isotype IgG
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Format Liquid
Description Anti-TCP1 beta Rabbit Monoclonal Antibody . Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Additional Information
Gene ID 10576
Other Names T-complex protein 1 subunit beta, TCP-1-beta, CCT-beta, Chaperonin containing T-complex polypeptide 1 subunit 2, CCT2, 99D8.1, CCTB
Calculated MW 57488 Da
Application Details WB 1:1000-1:5000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:50
Source Eukaryota
Contents Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clone Names Clone: 22C16
Immunogen A synthesized peptide derived from human TCP1 beta
Purification Affinity-chromatography
Storage Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name CCT2 {ECO:0000303|PubMed:25467444, ECO:0000312|HGNC:HGNC:1615}
Function Component of the chaperonin-containing T-complex (TRiC), a molecular chaperone complex that assists the folding of actin, tubulin and other proteins upon ATP hydrolysis (PubMed:25467444, PubMed:36493755, PubMed:35449234, PubMed:37193829). The TRiC complex mediates the folding of WRAP53/TCAB1, thereby regulating telomere maintenance (PubMed:25467444). As part of the TRiC complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia (PubMed:20080638).
Cellular Location Cytoplasm.
Research Areas
Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol
Blocking Peptides Protocol
Dot Blot Protocol
Immunohistochemistry Protocol
Immunofluorescence Protocol
Immunoprecipitation Protocol
Flow Cytomety Protocol
Cell Culture Protocol
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¥ 3,367.00
Cat# ABO15640
Size:
100 µl
Price:
¥ 3,367.00
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