Anti-CRYAA Rabbit Monoclonal Antibody

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF, ICC
Primary Accession	P02489
Host	Rabbit
Isotype	IgG
Reactivity	Rat, Human, Mouse
Clonality	Monoclonal
Format	Liquid
Description	Anti-CRYAA Rabbit Monoclonal Antibody . Tested in WB, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.

Additional Information
Gene ID	102724652;1409
Other Names	Alpha-crystallin A chain, Heat shock protein beta-4, HspB4, Heat shock protein family B member 4, Alpha-crystallin A(1-172), Alpha-crystallin A(1-168), Alpha-crystallin A(1-162), CRYAA, CRYA1, HSPB4
Calculated MW	19909 Da
Application Details	WB 1:500-1:2000 ICC/IF 1:50-1:200
Source	Eukaryota
Contents	Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clone Names	Clone: 23C19
Immunogen	A synthesized peptide derived from human CRYAA
Purification	Affinity-chromatography
Storage	Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	CRYAA
Synonyms	CRYA1, HSPB4
Function	Contributes to the transparency and refractive index of the lens (PubMed:18302245). In its oxidized form (absence of intramolecular disulfide bond), acts as a chaperone, preventing aggregation of various proteins under a wide range of stress conditions (PubMed:18199971, PubMed:19595763, PubMed:22120592, PubMed:31792453). Required for the correct formation of lens intermediate filaments as part of a complex composed of BFSP1, BFSP2 and CRYAA (PubMed:28935373).
Cellular Location	Cytoplasm. Nucleus. Note=Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles
Tissue Location	Expressed in the eye lens (at protein level).