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>   首页   >   产品   >   一抗   >   信号转导   >   Anti-S1PR3 Rabbit Monoclonal Antibody   

Anti-S1PR3 Rabbit Monoclonal Antibody

     
  • 1 - Anti-S1PR3 Rabbit Monoclonal Antibody ABO15826
    Figure 1. Western blot analysis of S1PR3 using anti-S1PR3 antibody (M03755).
    Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
    Lane 1: human HepG2 whole cell lysates,
    Lane 2: human Hela whole cell lysates,
    Lane 3: human A549 whole cell lysates,
    Lane 4: human SiHa whole cell lysates,
    Lane 5: rat C6 whole cell lysates,
    Lane 6: rat NRK whole cell lysates.
    After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-S1PR3 antigen affinity purified monoclonal antibody (Catalog # M03755) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for S1PR3 at approximately 42 kDa. The expected band size for S1PR3 is at 42 kDa.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB
Primary Accession Q99500
Host Rabbit
Isotype IgG
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Format Liquid
Description Anti-S1PR3 Rabbit Monoclonal Antibody . Tested in WB application. This antibody reacts with Human, Mouse, Rat.
Additional Information
Other Names Sphingosine 1-phosphate receptor 3, S1P receptor 3, S1P3, Endothelial differentiation G-protein coupled receptor 3, Sphingosine 1-phosphate receptor Edg-3, S1P receptor Edg-3, S1PR3 (HGNC:3167)
Calculated MW 42 KDa
Application Details WB 1:500-1:2000
Contents Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clone Names Clone: 24S03
Immunogen A synthesized peptide derived from human S1PR3
Purification Affinity-chromatography
Storage Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Research Areas
Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol
Blocking Peptides Protocol
Dot Blot Protocol
Immunohistochemistry Protocol
Immunofluorescence Protocol
Immunoprecipitation Protocol
Flow Cytomety Protocol
Cell Culture Protocol
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¥ 3,367.00
Cat# ABO15826
Size:
100 µl
Price:
¥ 3,367.00
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