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>   首页   >   产品   >   一抗   >   信号转导   >   Anti-FGF19 Rabbit Monoclonal Antibody   

Anti-FGF19 Rabbit Monoclonal Antibody

     
  • 1 - Anti-FGF19 Rabbit Monoclonal Antibody ABO15845
    Figure 1. Western blot analysis of FGF19 using anti-FGF19 antibody (M01191).
    Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
    Lane 1: human SH-SY5Y whole cell lysates,
    Lane 2: human U-87MG whole cell lysates,
    Lane 3: human CACO-2 whole cell lysates,
    Lane 4: human HepG2 whole cell lysates,
    Lane 5: rat liver tissue lysates,
    Lane 6: mouse liver tissue lysates.
    After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FGF19 antigen affinity purified monoclonal antibody (Catalog # M01191) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FGF19 at approximately 24 kDa. The expected band size for FGF19 is at 24 kDa.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB
Primary Accession O95750
Host Rabbit
Isotype IgG
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Format Liquid
Description Anti-FGF19 Rabbit Monoclonal Antibody . Tested in WB application. This antibody reacts with Human, Mouse, Rat.
Additional Information
Gene ID 9965
Other Names Fibroblast growth factor 19, FGF-19, FGF19
Calculated MW 24003 Da
Application Details WB 1:500-1:2000
Source Eukaryota
Contents Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clone Names Clone: 24F22
Immunogen A synthesized peptide derived from human FGF19
Purification Affinity-chromatography
Storage Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name FGF19
Function Involved in the suppression of bile acid biosynthesis through down-regulation of CYP7A1 expression, following positive regulation of the JNK and ERK1/2 cascades. Stimulates glucose uptake in adipocytes. Activity requires the presence of KLB and FGFR4.
Cellular Location Secreted.
Tissue Location Expressed in fetal brain, cartilage, retina, and adult gall bladder.
Research Areas
Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol
Blocking Peptides Protocol
Dot Blot Protocol
Immunohistochemistry Protocol
Immunofluorescence Protocol
Immunoprecipitation Protocol
Flow Cytomety Protocol
Cell Culture Protocol
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¥ 3,367.00
Cat# ABO15845
Size:
100 µl
Price:
¥ 3,367.00
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