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>   首页   >   产品   >   一抗   >   信号转导   >   Anti-ACAT2 Rabbit Monoclonal Antibody   

Anti-ACAT2 Rabbit Monoclonal Antibody

     
  • 1 - Anti-ACAT2 Rabbit Monoclonal Antibody ABO16147
    Figure 1. Western blot analysis of ACAT2 using anti-ACAT2 antibody (M03245-2).
    Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
    Lane 1: human K562 whole cell lysates,
    Lane 2: human HepG2 whole cell lysates,
    Lane 3: rat lung tissue lysates,
    Lane 4: rat kidney tissue lysates,
    Lane 5: mouse lung tissue lysates,
    Lane 6: mouse kidney tissue lysates.
    After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACAT2 antigen affinity purified monoclonal antibody (Catalog # M03245-2) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ACAT2 at approximately 41 kDa. The expected band size for ACAT2 is at 41 kDa.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC
Primary Accession Q9BWD1
Host Rabbit
Isotype IgG
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Format Liquid
Description Anti-ACAT2 Rabbit Monoclonal Antibody . Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.
Additional Information
Gene ID 39
Other Names Acetyl-CoA acetyltransferase, cytosolic, 2.3.1.9 {ECO:0000255|PROSITE-ProRule:PRU10020, ECO:0000269|PubMed:7911016}, Acetyl-CoA transferase-like protein, Cytosolic acetoacetyl-CoA thiolase, ACAT2, ACTL
Calculated MW 41351 Da
Application Details WB 1:500-1:2000
IHC 1:50-1:200
Source Eukaryota
Contents Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clone Names Clone: 27A26
Immunogen A synthesized peptide derived from human ACAT2
Purification Affinity-chromatography
Storage Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name ACAT2
Synonyms ACTL
Function Involved in the biosynthetic pathway of cholesterol.
Cellular Location Cytoplasm, cytosol.
Research Areas
Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol
Blocking Peptides Protocol
Dot Blot Protocol
Immunohistochemistry Protocol
Immunofluorescence Protocol
Immunoprecipitation Protocol
Flow Cytomety Protocol
Cell Culture Protocol
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¥ 3,367.00
Cat# ABO16147
Size:
100 µl
Price:
¥ 3,367.00
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ABO16147: Anti-ACAT2 Rabbit Monoclonal Antibody
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