Anti-SCIN Rabbit Monoclonal Antibody

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC
Primary Accession	Q9Y6U3
Host	Rabbit
Isotype	IgG
Reactivity	Rat, Human, Mouse
Clonality	Monoclonal
Format	Liquid
Description	Anti-SCIN Rabbit Monoclonal Antibody . Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.

Additional Information
Gene ID	85477
Other Names	Scinderin, Adseverin, SCIN {ECO:0000303\|PubMed:8547642, ECO:0000312\|HGNC:HGNC:21695}
Calculated MW	80489 Da
Application Details	WB 1:500-1:2000 IHC 1:50-1:200
Source	Eukaryota
Contents	Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clone Names	Clone: 27S73
Immunogen	A synthesized peptide derived from human SCIN
Purification	Affinity-chromatography
Storage	Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	SCIN {ECO:0000303\|PubMed:8547642, ECO:0000312\|HGNC:HGNC:21695}
Function	Ca(2+)-dependent actin filament-severing protein that has a regulatory function in exocytosis by affecting the organization of the microfilament network underneath the plasma membrane (PubMed:26365202, PubMed:8547642). Severing activity is inhibited by phosphatidylinositol 4,5-bis-phosphate (PIP2) (By similarity). In vitro, also has barbed end capping and nucleating activities in the presence of Ca(2+). Required for megakaryocyte differentiation, maturation, polyploidization and apoptosis with the release of platelet-like particles (PubMed:11568009). Plays a role in osteoclastogenesis (OCG) and actin cytoskeletal organization in osteoclasts (By similarity). Regulates chondrocyte proliferation and differentiation (By similarity). Inhibits cell proliferation and tumorigenesis. Signaling is mediated by MAPK, p38 and JNK pathways (PubMed:11568009).
Cellular Location	Cytoplasm, cytoskeleton. Cell projection, podosome {ECO:0000250\|UniProtKB:Q60604}
Tissue Location	Expressed in megakaryocytes.