购物车 0
注册 | 登录
0512-88856768
联系我们
点击这里给我发消息
所有产品
  • 所有产品
  • 一抗
  • 裂解液
>   首页   >   产品   >   一抗   >   信号转导   >   Anti-ERp19 Rabbit Monoclonal Antibody   

Anti-ERp19 Rabbit Monoclonal Antibody

     
  • 1 - Anti-ERp19 Rabbit Monoclonal Antibody ABO16199
    Figure 1. Western blot analysis of ERp19 using anti-ERp19 antibody (M08975).
    Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
    Lane 1: human 293T whole cell lysates,
    Lane 2: human THP-1 whole cell lysates,
    Lane 3: human HepG2 whole cell lysates,
    Lane 4: human U251 whole cell lysates,
    Lane 5: rat liver tissue lysates,
    Lane 6: mouse liver tissue lysates,
    Lane 7: mouse pancreas tissue lysates.
    After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ERp19 antigen affinity purified monoclonal antibody (Catalog # M08975) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ERp19 at approximately 17 kDa. The expected band size for ERp19 is at 19 kDa.
  • 产品详情
  • 实验流程
Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC, IF, ICC
Primary Accession O95881
Host Rabbit
Isotype IgG
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Format Liquid
Description Anti-ERp19 Rabbit Monoclonal Antibody . Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Additional Information
Gene ID 51060
Other Names Thioredoxin domain-containing protein 12, 1.8.4.2, Endoplasmic reticulum resident protein 18, ER protein 18, ERp18, Endoplasmic reticulum resident protein 19, ER protein 19, ERp19, Thioredoxin-like protein p19, hTLP19, TXNDC12 (HGNC:24626)
Calculated MW 19206 Da
Application Details WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
Source Eukaryota
Contents Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clone Names Clone: 27T78
Immunogen A synthesized peptide derived from human ERp19
Purification Affinity-chromatography
Storage Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name TXNDC12 (HGNC:24626)
Function Protein-disulfide reductase of the endoplasmic reticulum that promotes disulfide bond formation in client proteins through its thiol- disulfide oxidase activity.
Cellular Location Endoplasmic reticulum lumen {ECO:0000255|PROSITE- ProRule:PRU10138, ECO:0000269|PubMed:12761212}
Tissue Location Widely expressed..
Research Areas
Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol
Blocking Peptides Protocol
Dot Blot Protocol
Immunohistochemistry Protocol
Immunofluorescence Protocol
Immunoprecipitation Protocol
Flow Cytomety Protocol
Cell Culture Protocol
FeedBack

终于等到您。ABCEPTA（百远生物）抗体产品。
点击下方“我要评价 ”按钮提交您的反馈信息，您的反馈和评价是我们最宝贵的财富之一,
我们将在1-3个工作日内处理您的反馈信息。

如有疑问，联系：0512-88856768 tech-china@abcepta.com.


我要评价

¥ 3,367.00
Cat# ABO16199
Size:
100 µl
Price:
¥ 3,367.00
Quantity:
reduce add
Availability:
询价

相关产品

AP4871b: TXNDC12 Antibody (C-term)
ABO16199: Anti-ERp19 Rabbit Monoclonal Antibody
AP78798: ERp18 Rabbit mAb
查看更多产品
抗体制备服务
产品
新品速递
Crown抗体
一抗
产品文献引用
订单
联系我们
关于我们
新闻&活动
人才招聘
支持
技术支持
工具
相关链接
文献资料
促销活动
在线留言
客户售后反馈
Copyright © 2026 Abcepta Biotech Ltd. Co. 苏ICP备20009897号
地址：中国（江苏）自由贸易试验区苏州片区苏州工业园区双圩路9号1幢
电话：0512-88856768