Anti-PNK / PNKP Rabbit Monoclonal Antibody

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, IP
Primary Accession	Q96T60
Host	Rabbit
Isotype	IgG
Reactivity	Human
Clonality	Monoclonal
Format	Liquid
Description	Anti-PNK / PNKP Rabbit Monoclonal Antibody . Tested in WB, IHC, IP applications. This antibody reacts with Human.

Additional Information
Gene ID	11284
Other Names	Bifunctional polynucleotide phosphatase/kinase, DNA 5'-kinase/3'-phosphatase, Polynucleotide kinase-3'-phosphatase, Polynucleotide 3'-phosphatase, 3.1.3.32, 2'(3')-polynucleotidase, Polynucleotide 5'-hydroxyl-kinase, 2.7.1.78, PNKP {ECO:0000303\|PubMed:10446192, ECO:0000312\|HGNC:HGNC:9154}
Calculated MW	57076 Da
Application Details	WB 1:500-1:2000 IHC 1:50-1:200 IP 1:50
Source	Eukaryota
Contents	Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clone Names	Clone: 30P00
Immunogen	A synthesized peptide derived from human PNK / PNKP
Purification	Affinity-chromatography
Storage	Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	PNKP {ECO:0000303\|PubMed:10446192, ECO:0000312\|HGNC:HGNC:9154}
Function	Plays a key role in the repair of DNA damage, functioning as part of both the non-homologous end-joining (NHEJ) and base excision repair (BER) pathways (PubMed:10446192, PubMed:10446193, PubMed:15385968, PubMed:20852255, PubMed:28453785). Through its two catalytic activities, PNK ensures that DNA termini are compatible with extension and ligation by either removing 3'-phosphates from, or by phosphorylating 5'-hydroxyl groups on, the ribose sugar of the DNA backbone (PubMed:10446192, PubMed:10446193).
Cellular Location	Nucleus. Chromosome. Note=Localizes to site of double-strand breaks.
Tissue Location	Expressed in many tissues with highest expression in spleen and testis, and lowest expression in small intestine (PubMed:10446192). Expressed in higher amount in pancreas, heart and kidney and at lower levels in brain, lung and liver (PubMed:10446193)