Anti-NTH1 Rabbit Monoclonal Antibody

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, IF, ICC
Primary Accession	P78549
Host	Rabbit
Isotype	IgG
Reactivity	Human
Clonality	Monoclonal
Format	Liquid
Description	Anti-NTH1 Rabbit Monoclonal Antibody . Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human.

Additional Information
Gene ID	4913
Other Names	Endonuclease III-like protein 1 {ECO:0000255\|HAMAP-Rule:MF_03183}, hNTH1, 3.2.2.- {ECO:0000255\|HAMAP-Rule:MF_03183, ECO:0000269\|PubMed:9927729}, 4.2.99.18 {ECO:0000255\|HAMAP-Rule:MF_03183, ECO:0000269\|PubMed:9927729}, Bifunctional DNA N-glycosylase/DNA-(apurinic or apyrimidinic site) lyase {ECO:0000255\|HAMAP-Rule:MF_03183}, DNA glycosylase/AP lyase {ECO:0000255\|HAMAP-Rule:MF_03183}, NTHL1 {ECO:0000255\|HAMAP-Rule:MF_03183}, NTH1, OCTS3
Calculated MW	33570 Da
Application Details	WB 1:500-1:2000 IHC 1:50-1:200 ICC/IF 1:50-1:200
Source	Eukaryota
Contents	Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clone Names	Clone: 30N17
Immunogen	A synthesized peptide derived from human NTH1
Purification	Affinity-chromatography
Storage	Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	NTHL1 {ECO:0000255\|HAMAP-Rule:MF_03183}
Synonyms	NTH1, OCTS3
Function	Bifunctional DNA N-glycosylase with associated apurinic/apyrimidinic (AP) lyase function that catalyzes the first step in base excision repair (BER), the primary repair pathway for the repair of oxidative DNA damage (PubMed:29610152, PubMed:9927729). The DNA N-glycosylase activity releases the damaged DNA base from DNA by cleaving the N-glycosidic bond, leaving an AP site. The AP-lyase activity cleaves the phosphodiester bond 3' to the AP site by a beta- elimination. Primarily recognizes and repairs oxidative base damage of pyrimidines. Also has 8-oxo-7,8-dihydroguanine (8-oxoG) DNA glycosylase activity. Acts preferentially on DNA damage opposite guanine residues in DNA. Is able to process lesions in nucleosomes without requiring or inducing nucleosome disruption.
Cellular Location	Nucleus {ECO:0000255\|HAMAP-Rule:MF_03183, ECO:0000269\|PubMed:10882850, ECO:0000269\|PubMed:12531031, ECO:0000269\|PubMed:9611236}. Mitochondrion {ECO:0000255\|HAMAP- Rule:MF_03183, ECO:0000269\|PubMed:9611236}
Tissue Location	Widely expressed with highest levels in heart and lowest levels in lung and liver.