癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
JMJD2A Antibody
Rabbit Polyclonal Antibody
- 产品详情
- 实验流程
- 背景知识
Application 
| WB |
|---|---|
| Primary Accession | O75164 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 120662 Da |
| Positive Control | Western blot: DLD1 cell lysate |
|---|---|
| Application & Usage | Western blot: 1:200 dilution. Not recommended for FACS and IHC based on probable cross reactivity with other JMJD containing proteins. |
| Alias Symbol | JMJD2A |
| Other Names | Jumonji Domain Containing 2A; KDM4A; Lysine-Specific Demethylase 4A |
| Appearance | Colorless liquid |
| Formulation | 100 µl of antibody in TBS, pH 7.4, containing 50% glycerol, 0.5 mg/ml BSA, and 0.02% sodium azide. |
| Reconstitution & Storage | -20 °C |
| Background Descriptions | |
| Precautions | JMJD2A Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
For Research Use Only. Not For Use In Diagnostic Procedures.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
Jumonji Domain containing 2A (JMJD2A) is a lysine specific demethylase with emerging roles in histone modification or epigenetic remodeling. This JMJD2A polyclonal antibody was raised against an N-terminal recombinant fragment of JMJD2A. This fragment includes amino acids 1-350 including the JMJN and JMJC domains but not the two LAP/PHD zinc finger or Tudor domains of the 1,064 amino acid protein. JMJD2A is detected at 135 kDa by western blotting using a DLD1 cell lysate as a positive control. Also, the expression of JMJD2A varies by tissue, and northern blotting suggests skeletal muscle as a negative control and lung as a positive control tissue.
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