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>   首页   >   产品   >   一抗   >   癌症   >   His-Tag Monoclonal Antibody   

His-Tag Monoclonal Antibody

Mouse Monoclonal Antibody

     
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, E
Host Mouse
Clonality Monoclonal
Isotype Mouse IgG
Additional Information
Application & Usage ELISA, WB
Alias Symbol His
Other Names His-Tag
Appearance Colorless liquid
Formulation 100 ug (1mg/ml) of antibody in 0.01M Tris-HCl, pH 8.0, 0.15M NaCl, and 0.02% sodium azide.
Reconstitution & Storage -20 °C
Background Descriptions
PrecautionsHis-Tag Monoclonal Antibody is for research use only and not for use in diagnostic or therapeutic procedures.

For Research Use Only. Not For Use In Diagnostic Procedures.

Research Areas

BACKGROUND

A variety of plasmids contain DNA that encodes an N-terminal tag consisting of six histidine (His) residues, followed by an extended multiple cloning sites. The 6xHis tag on the expressed recombinant proteins allows for efficient coupling to Ni++ affinity resins and purification by a single step chromatography. As is the case with other protein tags, this polyhistidine tag can be cleaved at sites recognized by enzymes such as thrombin and enterokinases to isolate the protein of interest.

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