Anti-GLI1 Antibody

WB analysis of GLI1 expression in PC3 (A); mouse brain (B); rat brain (C) whole cell lysates.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB
Primary Accession	P08151
Reactivity	Human, Mouse, Rat
Host	Rabbit
Isotype	Rabbit IgG
Calculated MW	117904 Da

Additional Information
Gene ID	2735
Positive Control	WB: PC3, mouse barin, rat brain lysates
Application & Usage	WB; 1:500 – 1:2000
Other Names	s GLI; Zinc finger protein GLI1; Glioma-associated oncogene; Oncogene GLI
Target/Specificity	GLI1
Antibody Form	Liquid
Appearance	Colorless liquid
Handling	The antibody solution should be gently mixed before use
Reconstitution & Storage	-20°C
Background Descriptions
Precautions	Anti-GLI1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	GLI1
Synonyms	GLI
Function	Acts as a transcriptional activator (PubMed:10806483, PubMed:19706761, PubMed:19878745, PubMed:24076122, PubMed:24217340, PubMed:24311597). Binds to the DNA consensus sequence 5'-GACCACCCA-3' (PubMed:2105456, PubMed:24217340, PubMed:8378770). Regulates the transcription of specific genes during normal development (PubMed:19706761). Plays a role in craniofacial development and digital development, as well as development of the central nervous system and gastrointestinal tract. Mediates SHH signaling (PubMed:19706761, PubMed:28973407). Plays a role in cell proliferation and differentiation via its role in SHH signaling (PubMed:11238441, PubMed:28973407).
Cellular Location	Cytoplasm. Nucleus. Note=Tethered in the cytoplasm by binding to SUFU (PubMed:10806483). Activation and translocation to the nucleus is promoted by interaction with STK36 (PubMed:10806483). Phosphorylation by ULK3 may promote nuclear localization (PubMed:19878745). Translocation to the nucleus is promoted by interaction with ZIC1 (PubMed:11238441)
Tissue Location	Detected in testis (at protein level) (PubMed:2105456). Testis, myometrium and fallopian tube. Also expressed in the brain with highest expression in the cerebellum, optic nerve and olfactory tract (PubMed:19878745). Isoform 1 is detected in brain, spleen, pancreas, liver, kidney and placenta; isoform 2 is not detectable in these tissues (PubMed:19706761)