癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
Anti-Nitrotyrosine Antibody
Mouse Monoclonal Antibody
- 产品详情
- 实验流程
- 背景知识
Application 
| WB, IF, E |
|---|---|
| Reactivity | Human, Mouse, Rat |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | Mouse IgG1 |
| Clone Names | 6B2 |
| Antibody Form | Liquid |
|---|---|
| Appearance | Colorless liquid |
| Formulation | PBS, 10 mM NaCl 0.15 M pH 7.4– Thimerosal 0.01% may be used as preservative |
| Handling | The antibody solution should be gently mixed before use. |
| Reconstitution & Storage | -20 °C |
| Background Descriptions | |
| Precautions | Anti-Nitrotyrosine Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
For Research Use Only. Not For Use In Diagnostic Procedures.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
Oxidative stress has different functional consequences depending not only upon its intensity and duration, but also upon the nature of the free radicals, ROS or RNS, generated. Interestingly, the nature of these reactive species depends on the ratio between the initially produced ROS, superoxide anion (O2.-), and nitric oxide (NO). Indeed, O2.- very rapidly reacts with NO and therefore, as long as the NO/ O2.- ratio is ≥ 1, O2.- will therefore preferentially react with NO rather than with macromolecules, thus generating reactive nitrogen species (RNS): nitrosonium (NO+), N2O3 and peroxinitrite (ONOO-). These RNS induce posttranslational modifications: for NO+ and N2O3, S-nitrosation (Cys-SNO) and for ONOO-, tyrosine nitration (Tyr-NO2), methionine sulfoxidation (Met-SOH) and thiol oxidation (RS- S-R). When the O2.-/NO ratio becomes > 1, the O2.- and NO.2 ions and thereafter the OH. radicals, cause irreversible oxidations and peroxidations of macromolecules which generally lead to cell death(1-4). The monoclonal antibodies have been selected for their high affinity and specificity towards Tyr-NO2 residues. They do not cross-react with Tyr or Tyr derivatives such as aminotyrosine, chlorotyrosine or phosphotyrosine , neither with nitroTrp which can also be generated in response to peroxynitrite. They also recognize Tyr-NO2 residues in various sequences as shown by their ability to recognize nitrated proteins including albumin, ovalbumin, insulin, hemoglobin, KLH and various cytoplasmic and mitochondrial proteins.
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