E-Cadherin

Mouse Monoclonal antibody(Mab)

Immunohistochemical analysis of formalin-fixed and paraffin-embedded human breast tissue was performed with E-Cadherin primary antibody, followed by peroxidase-conjugated secondary antibody and DAB staining.
Immunohistochemical analysis of formalin-fixed and paraffin-embedded human prostatic adenocarcinoma tissue was performed with E-Cadherin primary antibody, followed by peroxidase-conjugated secondary antibody and DAB staining.
Immunohistochemical analysis of formalin-fixed and paraffin-embedded human ductal carcinoma of breast tissue was performed with E-Cadherin primary antibody, followed by peroxidase-conjugated secondary antibody and DAB staining.

产品详情
实验流程

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	IHC-P
Primary Accession	P12830
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Clone Names	499C4F1
Calculated MW	97456 Da

Additional Information
Gene ID	999
Gene Name	CDH1
Other Names	Cadherin-1, CAM 120/80, Epithelial cadherin, E-cadherin, Uvomorulin, CD324, E-Cad/CTF1, E-Cad/CTF2, E-Cad/CTF3, CDH1, CDHE, UVO
Dilution	IHC-P~~Pre-diluted Ready-To-Use (RTU)
Storage	Maintain refrigerated at 2-8°C.
Precautions	E-Cadherin Antibody is for research use only and not for use in diagnostic or therapeutic procedures.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	CDH1 (HGNC:1748)
Function	Cadherins are calcium-dependent cell adhesion proteins (PubMed:11976333). They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells (PubMed:11976333). Promotes organization of radial actin fiber structure and cellular response to contractile forces, via its interaction with AMOTL2 which facilitates anchoring of radial actin fibers to CDH1 junction complexes at the cell membrane (By similarity). Plays a role in the early stages of desmosome cell-cell junction formation via facilitating the recruitment of DSG2 and DSP to desmosome plaques (PubMed:29999492). Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7.
Cellular Location	Cell junction, adherens junction. Cell membrane; Single-pass type I membrane protein. Endosome. Golgi apparatus, trans-Golgi network. Cytoplasm. Cell junction, desmosome. Note=Colocalizes with DLGAP5 at sites of cell-cell contact in intestinal epithelial cells. Anchored to actin microfilaments through association with alpha-, beta- and gamma- catenin. Sequential proteolysis induced by apoptosis or calcium influx, results in translocation from sites of cell-cell contact to the cytoplasm. Colocalizes with RAB11A endosomes during its transport from the Golgi apparatus to the plasma membrane. Recruited to desmosomes at the initial assembly phase and also accumulates progressively at mature desmosome cell-cell junctions (PubMed:25208567, PubMed:29999492) Localizes to cell-cell contacts as keratinocyte differentiation progresses (By similarity). {ECO:0000250\|UniProtKB:P09803, ECO:0000269\|PubMed:25208567, ECO:0000269\|PubMed:29999492}
Tissue Location	Expressed in granuloma macrophages (at protein level) (PubMed:27760340). Expressed in the skin (at protein level) (PubMed:22294297). Expressed in the basal epithelial layer of the esophageal epithelium (at protein level) (PubMed:24220297). Expressed in the liver (PubMed:3263290).