Desmin Antibody

Mouse Monoclonal Antibody (Mab)

Immunohistochemical analysis of paraffin-embedded colorectal carcinoma; tissue using AD80025 performed on the Abcarta® FAIP-48 Fully automated IHC platform.Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a Citrate buffer (pH6. 0).Samples were incubated with primary antibody(Ready-to-use) for 15 min at room temperature. AmpSeeTM Detection Systems（Abcepta:ADR005） was used as the secondary antibody.

产品详情
实验流程

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	IHC
Primary Accession	P17661
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Isotype	IgG1
Clone Names	105A3E2
Calculated MW	53536 Da

Additional Information
Gene ID	1674
Gene Name	DES
Other Names	Desmin, DES
Dilution	IHC~~Ready-to-use
Storage	Maintain refrigerated at 2-8°C.
Precautions	Desmin Antibody is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	DES
Function	Muscle-specific type III intermediate filament essential for proper muscular structure and function. Plays a crucial role in maintaining the structure of sarcomeres, inter-connecting the Z-disks and forming the myofibrils, linking them not only to the sarcolemmal cytoskeleton, but also to the nucleus and mitochondria, thus providing strength for the muscle fiber during activity (PubMed:25358400). In adult striated muscle they form a fibrous network connecting myofibrils to each other and to the plasma membrane from the periphery of the Z- line structures (PubMed:24200904, PubMed:25394388, PubMed:26724190). May act as a sarcomeric microtubule-anchoring protein: specifically associates with detyrosinated tubulin-alpha chains, leading to buckled microtubules and mechanical resistance to contraction. Required for nuclear membrane integrity, via anchoring at the cell tip and nuclear envelope, resulting in maintenance of microtubule-derived intracellular mechanical forces (By similarity). Contributes to the transcriptional regulation of the NKX2-5 gene in cardiac progenitor cells during a short period of cardiomyogenesis and in cardiac side population stem cells in the adult. Plays a role in maintaining an optimal conformation of nebulette (NEB) on heart muscle sarcomeres to bind and recruit cardiac alpha-actin (By similarity).
Cellular Location	Cytoplasm, myofibril, sarcomere, Z line. Cytoplasm Cell membrane, sarcolemma. Nucleus {ECO:0000250\|UniProtKB:P31001}. Cell tip {ECO:0000250\|UniProtKB:P31001}. Nucleus envelope {ECO:0000250\|UniProtKB:P31001}. Note=Localizes in the intercalated disks which occur at the Z line of cardiomyocytes (PubMed:24200904, PubMed:26724190). Localizes in the nucleus exclusively in differentiating cardiac progenitor cells and premature cardiomyocytes (By similarity). PKP2 is required for correct anchoring of DES at the cell tip and nuclear envelope (By similarity) {ECO:0000250\|UniProtKB:P31001, ECO:0000269\|PubMed:24200904, ECO:0000269\|PubMed:26724190}

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol