CD22 Antibody

Mouse Monoclonal Antibody (Mab)

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	IHC
Primary Accession	P20273
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Isotype	IgG1
Clone Names	332A8D1
Calculated MW	95348 Da

Additional Information
Gene ID	933
Gene Name	CD22
Other Names	B-cell receptor CD22, B-lymphocyte cell adhesion molecule, BL-CAM, Sialic acid-binding Ig-like lectin 2, Siglec-2, T-cell surface antigen Leu-14, CD22, CD22, SIGLEC2
Dilution	IHC~~1:100~500
Storage	Maintain refrigerated at 2-8°C.
Precautions	CD22 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	CD22 {ECO:0000303\|PubMed:1691828, ECO:0000312\|HGNC:HGNC:1643}
Function	Most highly expressed siglec (sialic acid-binding immunoglobulin-like lectin) on B-cells that plays a role in various aspects of B-cell biology including differentiation, antigen presentation, and trafficking to bone marrow (PubMed:34330755, PubMed:8627166). Binds to alpha 2,6-linked sialic acid residues of surface molecules such as CD22 itself, CD45 and IgM in a cis configuration. Can also bind to ligands on other cells as an adhesion molecule in a trans configuration (PubMed:20172905). Acts as an inhibitory coreceptor on the surface of B-cells and inhibits B-cell receptor induced signaling, characterized by inhibition of the calcium mobilization and cellular activation. Mechanistically, the immunoreceptor tyrosine-based inhibitory motif domain is phosphorylated by the Src kinase LYN, which in turn leads to the recruitment of the protein tyrosine phosphatase 1/PTPN6, leading to the negative regulation of BCR signaling (PubMed:8627166). If this negative signaling from is of sufficient strength, apoptosis of the B-cell can be induced (PubMed:20516366).
Cellular Location	Cell membrane; Single-pass type I membrane protein
Tissue Location	B-lymphocytes.