p21/WAF1 Antibody

Mouse Monoclonal Antibody (Mab)

Immunohistochemical analysis of paraffin-embedded human cervical carcinoma tissue using AD80417 performed on the Abcarta® FAIP-48 Fully automated IHC platform.Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a Citrate buffer (pH6. 0).Samples were incubated with primary antibody(Ready-to-use) for 15 min at room temperature. AmpSeeTM Detection Systems（Abcepta:ADR005） was used as the secondary antibody.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	IHC
Primary Accession	P38936
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Clone Names	745E1B3
Calculated MW	18119 Da

Additional Information
Gene ID	1026
Gene Name	CDKN1A
Other Names	Cyclin-dependent kinase inhibitor 1, CDK-interacting protein 1, Melanoma differentiation-associated protein 6, MDA-6, p21, CDKN1A, CAP20, CDKN1, CIP1, MDA6, PIC1, SDI1, WAF1
Dilution	IHC~~Ready-to-use
Storage	Maintain refrigerated at 2-8°C.
Precautions	p21/WAF1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	CDKN1A (HGNC:1784)
Function	Plays an important role in controlling cell cycle progression and DNA damage-induced G2 arrest (PubMed:9106657). Involved in p53/TP53 mediated inhibition of cellular proliferation in response to DNA damage. Also involved in p53-independent DNA damage-induced G2 arrest mediated by CREB3L1 in astrocytes and osteoblasts (By similarity). Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex. Inhibits DNA synthesis by DNA polymerase delta by competing with POLD3 for PCNA binding (PubMed:11595739). Negatively regulates the CDK4- and CDK6-driven phosphorylation of RB1 in keratinocytes, thereby resulting in the release of E2F1 and subsequent transcription of E2F1-driven G1/S phase promoting genes (By similarity).
Cellular Location	Cytoplasm. Nucleus
Tissue Location	Expressed in all adult tissues, with 5-fold lower levels observed in the brain

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.