Anti-Digoxigenin antibody

Rabbit Monoclonal antibody(Mab)

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	IHC-P
Primary Accession	P10144
Reactivity	Human
Host	Rabbit
Clonality	Monoclonal
Clone Names	417M1A3
Calculated MW	27716 Da

Additional Information
Gene ID	3002
Other Names	Granzyme B, 3.4.21.79, C11, CTLA-1, Cathepsin G-like 1, CTSGL1, Cytotoxic T-lymphocyte proteinase 2, Lymphocyte protease, Fragmentin-2, Granzyme-2, Human lymphocyte protein, HLP, SECT, T-cell serine protease 1-3E, GZMB {ECO:0000303\|PubMed:32188940, ECO:0000312\|HGNC:HGNC:4709}
Dilution	IHC-P~~Ready-to-use
Storage	Maintain refrigerated at 2-8°C.

Protein Information
Name	GZMB {ECO:0000303\|PubMed:32188940, ECO:0000312\|HGNC:HGNC:4709}
Function	Abundant protease in the cytosolic granules of cytotoxic T- cells and NK-cells which activates caspase-independent pyroptosis when delivered into the target cell through the immunological synapse (PubMed:1985927, PubMed:3262682, PubMed:3263427). It cleaves after Asp (PubMed:1985927, PubMed:8258716). Once delivered into the target cell, acts by catalyzing cleavage of gasdermin-E (GSDME), releasing the pore- forming moiety of GSDME, thereby triggering pyroptosis and target cell death (PubMed:31953257, PubMed:32188940). Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -9 and -10 (CASP3, CASP9 and CASP10, respectively) to give rise to active enzymes mediating apoptosis (PubMed:9852092). Cleaves and activates CASP7 in response to bacterial infection, promoting plasma membrane repair (By similarity).
Cellular Location	Secreted. Cytolytic granule. Note=Delivered into the target cell by perforin (PubMed:20038786).