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>   首页   >   产品   >   一抗   >   精选抗体   >   Anti-Flotillin 1 Antibody (C-term), Biotinylated   

Anti-Flotillin 1 Antibody (C-term), Biotinylated

     
  • 1 - Anti-Flotillin 1 Antibody (C-term), Biotinylated AF4275a
    Biotinylated Antibody (1 µg/ml) staining of K562 lysate (35 µg protein in RIPA buffer), exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC, E
Primary Accession O75955
Other Accession 10211, NP_005794.1, NP_001305804.1
Reactivity Human
Predicted Human, Pig, Dog
Calculated MW 47355 Da
Additional Information
Gene ID 10211
Other Names Flotillin-1, FLOT1
Target/Specificity This antibody is expected to recognize both reported isoforms (NP_005794.1; NP_001305804.1).
Dilution WB~~1:1000
IHC~~1:100~500
E~~N/A
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsAnti-Flotillin 1 Antibody (C-term), Biotinylated is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name FLOT1
Function May act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles.
Cellular Location Cell membrane; Peripheral membrane protein. Endosome Membrane, caveola {ECO:0000250|UniProtKB:O08917}; Peripheral membrane protein {ECO:0000250|UniProtKB:O08917}. Melanosome. Membrane raft. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV (PubMed:17081065) Membrane-associated protein of caveola (By similarity) {ECO:0000250|UniProtKB:O08917, ECO:0000269|PubMed:17081065}
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

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