Goat anti-ACADM, Biotinylated Antibody

Peptide-affinity purified goat antibody

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, Pep-ELISA
Primary Accession	P11310
Other Accession	NP_000007.1, NP_001120800.1, NP_001272971.1, NP_001272972.1, NP_001272973.1
Reactivity	Human
Host	Goat
Clonality	Polyclonal
Clone Names	ACADM
Calculated MW	46588 Da

Additional Information
Gene ID	34
Other Names	ACADM; acyl-CoA dehydrogenase, C-4 to C-12 straight chain; ACAD1; MCAD; MCADH; acyl-Coenzyme A dehydrogenase, C-4 to C-12 straight chain
Dilution	WB~~1:1000 IHC~~1:100~500 Pep-ELISA~~N/A
Format	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Immunogen	This antibody is expected to recognise both reported isoforms (NP_000007.1; NP_001120800.1).
Storage	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	Goat anti-ACADM, Biotinylated Antibody is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	ACADM (HGNC:89)
Function	Medium-chain specific acyl-CoA dehydrogenase is one of the acyl-CoA dehydrogenases that catalyze the first step of mitochondrial fatty acid beta-oxidation (FAO), breaking down fatty acids into acetyl- CoA and allowing the production of energy from fats (PubMed:1970566, PubMed:21237683, PubMed:2251268, PubMed:8823175). The first step of FAO consists in the proR-proR stereospecific alpha, beta-dehydrogenation of fatty acyl-CoA thioesters using the electron transfer flavoprotein (ETF) as their physiologic electron acceptor, resulting in the formation of trans-2-enoyl-CoA ((2E)-enoyl-CoA) (PubMed:2251268). ETF is the electron acceptor that transfers electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase) (PubMed:15159392, PubMed:25416781). Among the different mitochondrial acyl-CoA dehydrogenases, medium-chain specific acyl-CoA dehydrogenase has preference for fatty acyl-CoAs with saturated 6 to 12 carbons long primary chains, making it but can also catalyze longer chains such as C14 and C16 (PubMed:1970566, PubMed:21237683, PubMed:2251268, PubMed:8823175).
Cellular Location	Mitochondrion matrix
Tissue Location	Expressed ubiquitously with highest levels in heart and muscle.