Goat anti-ACAT1 (aa253-266), Biotinylated Antibody

Peptide-affinity purified goat antibody

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF, Pep-ELISA
Primary Accession	P24752
Other Accession	NP_000010.1
Reactivity	Human, Mouse, Rat, Dog, Bovine
Host	Goat
Clonality	Polyclonal
Clone Names	ACAT1
Calculated MW	45200 Da

Additional Information
Gene ID	38
Other Names	ACAT1; acetyl-CoA acetyltransferase 1; ACAT; MAT; T2; THIL; acetoacetyl Coenzyme A thiolase; acetoacetyl-CoA thiolase; acetyl-Coenzyme A acetyltransferase 1; mitochondrial acetoacetyl-CoA thiolase
Dilution	WB~~1:1000 IF~~1:50~200 Pep-ELISA~~N/A
Format	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Storage	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	Goat anti-ACAT1 (aa253-266), Biotinylated Antibody is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	ACAT1
Synonyms	ACAT, MAT
Function	This is one of the enzymes that catalyzes the last step of the mitochondrial beta-oxidation pathway, an aerobic process breaking down fatty acids into acetyl-CoA (PubMed:1715688, PubMed:7728148, PubMed:9744475). Using free coenzyme A/CoA, catalyzes the thiolytic cleavage of medium- to long-chain 3-oxoacyl-CoAs into acetyl-CoA and a fatty acyl-CoA shortened by two carbon atoms (PubMed:1715688, PubMed:7728148, PubMed:9744475). The activity of the enzyme is reversible and it can also catalyze the condensation of two acetyl-CoA molecules into acetoacetyl-CoA (PubMed:17371050). Thereby, it plays a major role in ketone body metabolism (PubMed:1715688, PubMed:17371050, PubMed:7728148, PubMed:9744475).
Cellular Location	Mitochondrion.