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Goat Anti-VGLUT1 Antibody

Peptide-affinity purified goat antibody

EB08600 (3.75µg/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB08600 (0.25µg/ml) staining of paraffin embedded Mouse Hypothalamus and Thalamus. Antigen retrieval at 80C for 30min with citrate buffer pH 6, HRP-staining (data kindly provided by Dr. E. Hrabovszky, Budapest, Hungary
EB08600 (3.75µg/ml) staining of paraffin embedded Human Adrenal Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

产品详情
实验流程

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	IHC, IF, Pep-ELISA
Primary Accession	Q9P2U7
Other Accession	NP_064705.1
Reactivity	Human, Mouse, Rat, Dog, Bovine
Host	Goat
Clonality	Polyclonal
Clone Names	SLC17A7
Calculated MW	61613 Da

Additional Information
Gene ID	57030
Other Names	SLC17A7, solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 7, BNPI, VGLUT1, brain-specific Na-dependent inorganic phosphate cotransporter, solute carrier family 17, member 7, vesicular glutamate transporter 1
Dilution	IHC~~1:100~500 IF~~1:50~200 Pep-ELISA~~N/A
Format	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Immunogen	Peptide with sequence C-HDQLAGSDDSEMED, from the internal region of the protein sequence according to NP_064705.1.
Storage	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	Goat Anti-VGLUT1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	SLC17A7 (HGNC:16704)
Function	Multifunctional transporter that transports L-glutamate as well as multiple ions such as chloride, proton, potassium, sodium and phosphate (PubMed:10820226). At the synaptic vesicle membrane, mainly functions as an uniporter which transports preferentially L-glutamate but also phosphate from the cytoplasm into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells (By similarity). The L-glutamate or phosphate uniporter activity is electrogenic and is driven by the proton electrochemical gradient, mainly by the electrical gradient established by the vacuolar H(+)-ATPase across the synaptic vesicle membrane (By similarity). In addition, functions as a chloride channel that allows a chloride permeation through the synaptic vesicle membrane that affects the proton electrochemical gradient and promotes synaptic vesicles acidification (By similarity). Moreover, may function as a K(+)/H(+) antiport allowing to maintain the electrical gradient and to decrease chemical gradient and therefore sustain vesicular glutamate uptake (By similarity). The vesicular K(+)/H(+) antiport activity is electroneutral (By similarity). At the plasma membrane, following exocytosis, functions as a symporter of Na(+) and phosphate from the extracellular space to the cytoplasm allowing synaptic phosphate homeostasis regulation (PubMed:10820226). The symporter activity is driven by an inside negative membrane potential and is electrogenic (By similarity). Is necessary for synaptic signaling of visual-evoked responses from photoreceptors (By similarity).
Cellular Location	Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane {ECO:0000250\|UniProtKB:Q3TXX4}. Cell membrane; Multi-pass membrane protein. Synapse, synaptosome {ECO:0000250\|UniProtKB:Q3TXX4}
Tissue Location	Expressed in several regions of the brain including amygdala, cerebellum, cerebral cortex, hippocampus, frontal lobe, medulla, occipital lobe, putamen and temporal lobe

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol