癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
> 首页 > 产品 > 一抗 > 癌症 > CD31 / PECAM-1 (Endothelial Cell Marker)
Mouse Monoclonal Antibody [Clone C31.7]
CD31 / PECAM-1 (Endothelial Cell Marker) Mouse Monoclonal Antibody [Clone C31.7]
Purified Mouse Monoclonal Antibody
![1 - CD31 / PECAM-1 (Endothelial Cell Marker)
Mouse Monoclonal Antibody [Clone C31.7] AH10373](/assets/uploads/products/201506/AH10373_FC_1.jpg)
- 产品详情
- 实验流程
Application 
| IF, FC |
|---|---|
| Primary Accession | P16284 |
| Reactivity | Human, Rabbit, Monkey |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1, kappa |
| Clone Names | C31.7 |
| Calculated MW | 82522 Da |
| Gene ID | 5175 |
|---|---|
| Other Names | EndoCAM; PECA1; Platelet Endothelial Cell Adhesion Molecule 1; GPIIA' |
| Target/Specificity | Recombinant full-length human CD31 protein |
| Application Note | Flow Cytometry (5ul per test per one million cells or 5ul per 100ul of whole blood); Immunofluorescence (1:50-1:100 for 30 minutes at RT); (Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 1mM EDTA, pH 8.0, for 10-20 min followed by cooling at RT for 20 minutes) Optimal dilution for a specific application should be determined. |
| Format | 0.5ml at 100ug/ml with BSA and azide |
| Storage | Store at 2 to 8°C.Antibody is stable for 24 months. |
| Precautions | CD31 / PECAM-1 (Endothelial Cell Marker) Mouse Monoclonal Antibody [Clone C31.7] is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | PECAM1 |
|---|---|
| Function | Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions (PubMed:17580308, PubMed:19342684). Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes (PubMed:19342684). Trans-homophilic interaction may play a role in endothelial cell-cell adhesion via cell junctions (PubMed:27958302). Heterophilic interaction with CD177 plays a role in transendothelial migration of neutrophils (PubMed:17580308). Homophilic ligation of PECAM1 prevents macrophage-mediated phagocytosis of neighboring viable leukocytes by transmitting a detachment signal (PubMed:12110892). Promotes macrophage-mediated phagocytosis of apoptotic leukocytes by tethering them to the phagocytic cells; PECAM1-mediated detachment signal appears to be disabled in apoptotic leukocytes (PubMed:12110892). Modulates bradykinin receptor BDKRB2 activation (PubMed:18672896). Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in endothelial cells (PubMed:18672896). Induces susceptibility to atherosclerosis (By similarity). |
| Cellular Location | Cell membrane; Single-pass type I membrane protein. Note=Cell surface expression on neutrophils is down-regulated upon fMLP or CXCL8/IL8- mediated stimulation. [Isoform Delta15]: Cell junction. Note=Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells |
| Tissue Location | Expressed on platelets and leukocytes and is primarily concentrated at the borders between endothelial cells (PubMed:18388311, PubMed:21464369). Expressed in human umbilical vein endothelial cells (HUVECs) (at protein level) (PubMed:17580308, PubMed:19342684). Expressed on neutrophils (at protein level) (PubMed:17580308). Isoform Long predominates in all tissues examined (PubMed:12433657). Isoform Delta12 is detected only in trachea (PubMed:12433657). Isoform Delta14-15 is only detected in lung (PubMed:12433657). Isoform Delta14 is detected in all tissues examined with the strongest expression in heart (PubMed:12433657). Isoform Delta15 is expressed in brain, testis, ovary, cell surface of platelets, human umbilical vein endothelial cells (HUVECs), Jurkat T- cell leukemia, human erythroleukemia (HEL) and U-937 histiocytic lymphoma cell lines (at protein level) (PubMed:12433657, PubMed:18388311). |
Research Areas
For Research Use Only. Not For Use In Diagnostic Procedures.
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
REFERENCES
1. Gratzinger D et. al. Am J Clin Pathol 131:264-278 (2009).
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