癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
CD8A (Anti-Mouse) (Cytotoxic / Suppressor T-Cell Marker) Antibody - With BSA and Azide
Rat Monoclonal Antibody [Clone IBL-3/25 ]
- 产品详情
- 实验流程
- 背景知识
Application 
| IF, FC |
|---|---|
| Primary Accession | P01731 |
| Other Accession | 12525 (Mouse), 1858 (Mouse) |
| Reactivity | Mouse |
| Host | Rat |
| Clonality | Monoclonal |
| Isotype | Rat / IgG1, kappa |
| Clone Names | IBL-3/25 |
| Calculated MW | 27456 Da |
| Gene ID | 12525 |
|---|---|
| Other Names | T-cell surface glycoprotein CD8 alpha chain, T-cell surface glycoprotein Lyt-2, CD8a, Cd8a, Lyt-2, Lyt2 |
| Application Note | IF~~1:50~200 FC~~1:10~50 |
| Format | 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml. |
| Storage | Store at 2 to 8°C.Antibody is stable for 24 months. |
| Precautions | CD8A (Anti-Mouse) (Cytotoxic / Suppressor T-Cell Marker) Antibody - With BSA and Azide is for research use only and not for use in diagnostic or therapeutic procedures. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | Cd8a |
|---|---|
| Synonyms | Lyt-2, Lyt2 |
| Function | Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class I molecule:peptide complex. The antigens presented by class I peptides are derived from cytosolic proteins while class II derived from extracellular proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class I proteins presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of cytotoxic T- lymphocytes (CTLs). This mechanism enables CTLs to recognize and eliminate infected cells and tumor cells. In NK-cells, the presence of CD8A homodimers at the cell surface provides a survival mechanism allowing conjugation and lysis of multiple target cells. CD8A homodimer molecules also promote the survival and differentiation of activated lymphocytes into memory CD8 T-cells. |
| Cellular Location | Cell membrane {ECO:0000250|UniProtKB:P01732}; Single-pass type I membrane protein {ECO:0000250|UniProtKB:P01732} Note=Cd8a localizes to lipid rafts only when associated with its partner Cd8b. {ECO:0000250|UniProtKB:P01732} |
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
Recognizes a protein of 32kDa, identified as CD8a (also known as CD8 chain, T cell co-receptor). CD8 molecule consists of two chains, termed and chain, which are expressed as a disulphide-linked heterodimer or as an homodimer. CD8 is expressed on T cell subset (cytotoxic/suppressor T cells), thymocytes and NK cells. The majority of CD8+ T-cells expresses CD8 as heterodimer. Some subpopulation of CD8+ T cells as well as NK cells may express homodimer. CD8 functions as a co-receptor in concert with TCR for binding the MHC class I/peptide complex. The HIV-2 envelope glycoprotein binds CD8 chain (but not chain). The cytoplasmic domain of CD8 associates with p56lck tyrosine kinase.
REFERENCES
Knapp W. et. al. Leukocyte Typing IV, p342-343, Oxford University Press, 1989 Parnes JR, CD4 and CD8 in T cell lineage commitment: alterations induced by expression of a CD8/CD4 chimeric transgene. Semin Immunol 1994, 6:221-229. Leahy DJ. A structural view of CD4 and CD8. FASEB J. 1995,9(1):17-25
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