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>   首页   >   产品   >   一抗   >   其他   >    Microphthalmia Transcription Factor (MITF) Antibody - With BSA and Azide   

Microphthalmia Transcription Factor (MITF) Antibody - With BSA and Azide

Mouse Monoclonal Antibody [Clone MITF/915 ]

     
  • 2 -  Microphthalmia Transcription Factor (MITF) Antibody - With BSA and Azide AH11837
    Formalin-fixed, paraffin-embedded human Melanoma stained with MITF Monoclonal Antibody (MITF/915).
Formalin-fixed, paraffin-embedded human Melanoma stained with MITF Monoclonal Antibody (MITF/915).
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC, IF, FC
Primary Accession O75030
Other Accession 4286, 166017, 618266
Reactivity Human, Drosophila
Host Mouse
Clonality Monoclonal
Isotype Mouse / IgG1, kappa
Clone Names MITF/915
Calculated MW 58795 Da
Additional Information
Gene ID 4286
Other Names Microphthalmia-associated transcription factor, Class E basic helix-loop-helix protein 32, bHLHe32, MITF, BHLHE32
Application Note IHC~~1:100~500
IF~~1:50~200
FC~~1:10~50
StorageStore at 2 to 8°C.Antibody is stable for 24 months.
Precautions Microphthalmia Transcription Factor (MITF) Antibody - With BSA and Azide is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MITF {ECO:0000303|PubMed:8069297, ECO:0000312|HGNC:HGNC:7105}
Function Transcription factor that acts as a master regulator of melanocyte survival and differentiation as well as melanosome biogenesis (PubMed:10587587, PubMed:22647378, PubMed:27889061, PubMed:9647758). Binds to M-boxes (5'-TCATGTG-3') and symmetrical DNA sequences (E-boxes) (5'-CACGTG-3') found in the promoter of pigmentation genes, such as tyrosinase (TYR) (PubMed:10587587, PubMed:22647378, PubMed:27889061, PubMed:9647758). Involved in the cellular response to amino acid availability by acting downstream of MTOR: in the presence of nutrients, MITF phosphorylation by MTOR promotes its inactivation (PubMed:36608670). Upon starvation or lysosomal stress, inhibition of MTOR induces MITF dephosphorylation, resulting in transcription factor activity (PubMed:36608670). Plays an important role in melanocyte development by regulating the expression of tyrosinase (TYR) and tyrosinase-related protein 1 (TYRP1) (PubMed:10587587, PubMed:22647378, PubMed:27889061, PubMed:9647758). Plays a critical role in the differentiation of various cell types, such as neural crest-derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium (PubMed:10587587, PubMed:22647378, PubMed:27889061, PubMed:9647758).
Cellular Location Nucleus. Cytoplasm. Lysosome membrane Note=When nutrients are present, recruited to the lysosomal membrane via association with GDP-bound RagC/RRAGC (or RagD/RRAGD): it is then phosphorylated by MTOR (PubMed:23401004, PubMed:36608670) Phosphorylation by MTOR promotes ubiquitination and degradation (PubMed:36608670). Conversely, inhibition of mTORC1, starvation and lysosomal disruption, promotes dephosphorylation and translocation to the nucleus (PubMed:36608670). Phosphorylation by MARK3/cTAK1 promotes association with 14-3-3/YWHA adapters and retention in the cytosol (PubMed:16822840).
Tissue Location Expressed in melanocytes (at protein level). [Isoform C2]: Expressed in the kidney and retinal pigment epithelium. [Isoform H2]: Expressed in the kidney. [Isoform Mdel]: Expressed in melanocytes.
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

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