癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
Neurofilament, phospho (NF-H) (Neuronal Marker) Antibody - With BSA and Azide
Mouse Monoclonal Antibody [Clone NE14 ]
- 产品详情
- 实验流程
- 背景知识
Application 
| IHC, FC |
|---|---|
| Primary Accession | P12036 |
| Other Accession | 4744, 198760 |
| Reactivity | Human, Mouse, Rat, Rabbit, Pig, Chicken, Bovine, Cat, Guinea Pig, Gerbil |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | Mouse / IgG1 |
| Clone Names | NE14 |
| Calculated MW | 111838 Da |
| Gene ID | 4744 |
|---|---|
| Other Names | Neurofilament heavy polypeptide, NF-H, 200 kDa neurofilament protein, Neurofilament triplet H protein, NEFH, KIAA0845, NFH |
| Application Note | IHC~~1:100~500 FC~~1:10~50 |
| Storage | Store at 2 to 8°C.Antibody is stable for 24 months. |
| Precautions | Neurofilament, phospho (NF-H) (Neuronal Marker) Antibody - With BSA and Azide is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | NEFH |
|---|---|
| Synonyms | KIAA0845, NFH |
| Function | Neurofilaments usually contain three intermediate filament proteins: NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. NEFH has an important function in mature axons that is not subserved by the two smaller NF proteins. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks (By similarity). |
| Cellular Location | Cytoplasm, cytoskeleton. Cell projection, axon {ECO:0000250|UniProtKB:P19246} |
For Research Use Only. Not For Use In Diagnostic Procedures.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
This MAb reacts with a 200kDa protein, identified as heavy sub-unit of neurofilaments (NF-H). It reacts specifically with the phosphorylated KSP/KEP segment at the C-terminus of the heavy subunit (NF-H) of neurofilaments. After dephosphorylation of neurofilaments with alkaline phosphatase, this Ab no longer binds. Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68kDa (NF-L), 160kDa (NF-M) and 200kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilament.
REFERENCES
Debus E., et al. Differentiation 25(2), 193-203 (1983). | Gotow T., et al. J. Neuroscience Res. 37(6), 691-713 (1994). | Berglund A.M., et al. J Comp Neurol. 306(3), 393-408 (1991). | Guadano-Ferraz A., , et al. Develop. Brain Res. GOTOBUTTON BM_4_ 56(2), 244-256, (1990). | Obermann W., et al. Eur. J. Biochem 233, 110-115 (1995). | Marx A., et al. Am. J.Pathol. 148(6), 1839-1850 (1996)
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