癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
Anti-Desmoglein-1 (DSG1) Antibody
Mouse Monoclonal Antibody
- 产品详情
- 实验流程
- 背景知识
Application 
| WB, IF, FC |
|---|---|
| Primary Accession | Q02413 |
| Other Accession | 2633 |
| Reactivity | Human |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | Mouse / IgG1, kappa |
| Clone Names | 18D4 |
| Calculated MW | 113748 Da |
| Gene ID | 1828 |
|---|---|
| Other Names | Cadherin family member 4; CDHF4; Desmoglein-1; Desmosomal glycoprotein 1; DG1; DSG1; EPKHE; EPKHIA; Pemphigus foliaceus antigen; PPKS1; SPPK1 |
| Application Note | Flow Cytometry (0.5-1ug/million cells); ,Immunofluorescence (1-2ug/ml); ,Western Blotting (0.5-1.0ug/ml) ,Optimal dilution for a specific application should be determined. |
| Format | 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml. |
| Storage | Store at 2 to 8°C.Antibody is stable for 24 months. |
| Precautions | Anti-Desmoglein-1 (DSG1) Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | DSG1 |
|---|---|
| Synonyms | CDHF4 |
| Function | A component of desmosome cell-cell junctions which are required for positive regulation of cellular adhesion (PubMed:19717567). Promotes differentiation of keratinocytes via interaction with ERBIN. Reduces activation of RAS/ERK signaling pathways in keratinocytes via promoting ERBIN localization to the cell membrane and its interaction with SHOC2, this competitively reduces the formation of RAS-SHOC2 ERK-activating complexes (PubMed:23524970). Mediates the interaction between the desmosome and COP9 signalosome complex (CSN) protein complex (PubMed:28891468). As a result of this interaction, promotes keratinocyte differentiation via deneddylation of EGFR resulting in a reduction in EGFR protein stabilization and translocation away from the cell membrane (PubMed:28891468). Critical for epidermal integrity and barrier function of the skin (By similarity). Required for esophageal epithelial barrier integrity and correct intercellular spacing in differentiated esophageal epithelium (PubMed:24220297). Required for recovery of epidermal architecture and correct expression of differentiation markers following UVB exposure (PubMed:24594668). May also be required for OCLN involvement in tight junctions (By similarity). |
| Cellular Location | Cell membrane; Single-pass type I membrane protein {ECO:0000250|UniProtKB:Q7TSF1}. Cell junction, desmosome. Cytoplasm. Nucleus |
| Tissue Location | Expressed in all suprabasal layers of the epidermis, with the highest expression seen in the granular layer (at protein level). Expressed in the suprabasal esophageal epithelium (at protein level) (PubMed:24220297). |
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
Recognizes a protein of ~150kDa, identified as Desmoglein-1 (DSG1). Desmoglein-1 is a member of the desmosomal cadherin family. Desmosomes are intercellular adhering junctions that represent cell surface attachment sites for intermediate filament. Desmocollins and desmogleins are the main desmosomal transmembrane proteins. Desmogleins consist of Dsg1, Dsg2, Dsg3, and Dsg4 isoforms. Within the desmosome, the extracellular domain of desmoglein is essential for calcium dependent heterophilic binding to the desmocollins, whereas the intracellular domain is essential for binding to the desmosomal plaque protein, plakoglobin. Desmoglein 1 is synthesized exclusively in the suprabasal layers. Intact and functionally active desmoglein 1 is essential to epidermal integrity.
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