癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
Anti-Plakophilin-1 (PKP1) Antibody
Mouse Monoclonal Antibody
- 产品详情
- 实验流程
- 背景知识
Application 
| WB, IF, FC |
|---|---|
| Primary Accession | Q13835 |
| Other Accession | 497350 |
| Reactivity | Human, Mouse |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | Mouse / IgG1 |
| Clone Names | 10B2 |
| Calculated MW | 82861 Da |
| Gene ID | 5317 |
|---|---|
| Other Names | B6P; Band 6 protein; PKP1; Plakophilin-1 (ectodermal dysplasia/skin fragility syndrome) |
| Application Note | Flow Cytometry (0.5-1ug/million cells); ,Immunofluorescence (1-2ug/ml); ,Western Blotting (0.5-1.0ug/ml),Optimal dilution for a specific application should be determined. |
| Format | 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml. |
| Storage | Store at 2 to 8°C.Antibody is stable for 24 months. |
| Precautions | Anti-Plakophilin-1 (PKP1) Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | PKP1 |
|---|---|
| Function | A component of desmosome cell-cell junctions which are required for positive regulation of cellular adhesion (PubMed:23444369). Plays a role in desmosome protein expression regulation and localization to the desmosomal plaque, thereby maintaining cell sheet integrity and anchorage of desmosomes to intermediate filaments (PubMed:10852826, PubMed:23444369). Required for localization of DSG3 and YAP1 to the cell membrane in keratinocytes in response to mechanical strain, via the formation of an interaction complex composed of DSG3, YAP1, PKP1 and YWHAG (PubMed:31835537). Positively regulates differentiation of keratinocytes, potentially via promoting localization of DSG1 at desmosome cell junctions (By similarity). Required for calcium-independent development and maturation of desmosome plaques specifically at lateral cell-cell contacts in differentiating keratinocytes (By similarity). Plays a role in the maintenance of DSG3 protein abundance, DSG3 clustering and localization of these clusters to the cell membrane in keratinocytes (By similarity). May also promote keratinocyte proliferation and morphogenesis during postnatal development (PubMed:9326952). Required for tight junction inside-out transepidermal barrier function of the skin (By similarity). Promotes Wnt-mediated proliferation and differentiation of ameloblasts, via facilitating TJP1/ZO-1 localization to tight junctions (By similarity). Binds single-stranded DNA (ssDNA), and may thereby play a role in sensing DNA damage and promoting cell survival (PubMed:20613778). Positively regulates cap-dependent translation and as a result cell proliferation, via recruitment of EIF4A1 to the initiation complex and promotion of EIF4A1 ATPase activity (PubMed:20156963, PubMed:23444369). Regulates the mRNA stability and protein abundance of desmosome components PKP2, PKP3, DSC2 and DSP, potentially via its interaction with FXR1 (PubMed:25225333). |
| Cellular Location | [Isoform 1]: Cell junction, desmosome Nucleus. Cytoplasm, perinuclear region. Cytoplasm. Cell junction, desmosome. Cell membrane Cytoplasm, Stress granule Note=Colocalizes with EIF4A1 in stress granules following arsenate or hydrogen peroxide treatment (PubMed:20156963). Localizes to nucleoli following DNA damage (PubMed:20613778). Located in the cytoplasm during early tooth development, however localizes to the cell membrane in ameloblasts during molar growth (By similarity). Ca(2+)-mediated localization to the cell membrane in dental epithelial cells is inhibited via WNT3A (By similarity). Localizes to the cytoplasm when the phosphorylated form interacts with YWHAG (By similarity). Initially localized to the cytoplasm however as keratinocyte differentiation proceeds becomes localized to cell junctions as early cell-cell contacts become linear as part of membrane sealing (By similarity) Localized to lateral cell contacts in colocalization with DSP as epithelial sheet formation completes (By similarity). Protein stability is increased and localizes to the cytoplasm when phosphorylated at the N-terminus by AKT2 (PubMed:23444369). The unphosphorylated form is preferentially localized to desmosomes (PubMed:23444369) {ECO:0000250|UniProtKB:P97350, ECO:0000269|PubMed:20156963, ECO:0000269|PubMed:20613778, ECO:0000269|PubMed:23444369} |
| Tissue Location | [Isoform 1]: Expressed in stratified squamous, complex, glandular duct and bladder epithelia (at protein level) |
Research Areas
For Research Use Only. Not For Use In Diagnostic Procedures.
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
Recognizes a protein of 75kDa, identified as Plakophilin-1. Its epitope maps between aa 1-27 human Plakophilin-1. Plakophilins 1, 2, 3 and 4 (PKP1-4) influence development and participate in linking cadherins to cytoskeletal intermediate filaments. Plakophilins 1-4 contain arm-repeat (armadillo) domains and localize to nuclei and cell desmosomes (cell-cell junctions found in suprabasal layers of stratifying epithelia that undergo mechanical stress). Plakophilin 2 is important for desmosome assembly and is an essential morphogenic factor and architectural component of the heart.
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¥ 2,709.00
Cat# AH13436
