E. coli O157 Antibody
Mouse Monoclonal Antibody
- 产品详情
- 实验流程
- 背景知识
Application
| IF, E |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | Mouse / IgM, kappa |
| Clone Names | 9.88 |
| Other Names | E. coli O157Human Gene SymbolNot ApplicableHu Chromosome LocationNot Applicable |
|---|---|
| Application Note | ELISA (For coating use Ab at 1-5ug/ml, order Ab without BSA);Immunofluorescence (1-2ug/ml); Optimal dilution for a specific application should be determined. |
| Format | 200ug/ml of Ab purified from Bioreactor Concentrate. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml. |
| Storage | Store at 2 to 8°C.Antibody is stable for 24 months. |
| Precautions | E. coli O157 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
For Research Use Only. Not For Use In Diagnostic Procedures.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
This MAb shows specificity to E.coli O157 in a simple ELISA. Escherichia coli are Gram negative bacterium that are commonly found in the lower intestine of warm-blooded organisms (endotherms). Their serological types are determined in combination with somatic antigens (O group: O1-O173) and flagella antigens (H type: H1-H56). The E. coli that cause intestinal infectious diseases including diarrhea, acute gastritis or colitis are referred to as pathogenic E. coli, which are classified into the following 4 groups according to differences in the mode of pathogenicity; enteropathogenic E. coli (EPEC), enteroinvasive E. coli (EIEC), enterotoxigenic E. coli (ETEC) and enterohemorrhagic E. coli (EHEC). Although the identification of pathogenic E. coli requires verification of their pathogenicity, pathogenic E. coli often have specific serotypes; therefore, typing of the serogroup and serotype is necessary in screening pathogenic E. coli.
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