Anti-FITC (Fluorescein Isothiocyanate) Antibody
Mouse Monoclonal Antibody
- 产品详情
- 实验流程
- 背景知识
Application ![]()
| WB, IHC-P, IF, FC |
---|---|
Host | Mouse |
Clonality | Monoclonal |
Isotype | Mouse / IgG1 |
Clone Names | SPM395 |
Other Names | Fluorescein isothiocyanate, FITC |
---|---|
Application Note | Confocal Microscopy;Flow Cytometry (0.5-1ug/million cells); Immunofluorescence (0.5-1ug/ml); In situ Nucleic Acid Hybridization; Western Blotting;Immunohistology (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT) (Pretreatment depends upon the fluoreceinated primary antibody.)Optimal dilution for a specific application should be determined. |
Format | 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml. |
Storage | Store at 2 to 8°C.Antibody is stable for 24 months. |
Precautions | Anti-FITC (Fluorescein Isothiocyanate) Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
For Research Use Only. Not For Use In Diagnostic Procedures.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
This MAb recognizes both the free and protein-conjugated (either soluble or cell bound) form of fluorescein or carboxy-fluorescein. Sensitivity of the FITC-anti-FITC system is reportedly similar to that of the biotin-avidin system. In biotin or enzyme-labeled form, this MAb is also suitable for the conversation of fluorescein-labeled detection into immuno-enzymatic assays.

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