癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
Anti-Cytokeratin, Acidic (Type I or LMW) Antibody
Mouse Monoclonal Antibody
- 产品详情
- 实验流程
- 背景知识
Application 
| WB, IHC-P, IF, FC |
|---|---|
| Primary Accession | Q7Z794 |
| Other Accession | 334989 |
| Reactivity | Human |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | Mouse / IgG1 |
| Clone Names | KRTL/1377 |
| Calculated MW | 61901 Da |
| Gene ID | 374454 |
|---|---|
| Other Names | K1B; KRT1B; K77; CK-1B; Keratin 1B; Keratin-77; Cytokeratin-1B |
| Application Note | Flow Cytometry (0.5-1ug/million cells); ,Immunofluorescence (1-2ug/ml); Western Blotting (0.5-1ug/ml); ,Immunohistology (Formalin-fixed) (1-2ug/ml for 30 min at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined. |
| Format | 200ug/ml of Ab purified from bioreactor concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml. |
| Storage | Store at 2 to 8°C.Antibody is stable for 24 months. |
| Precautions | Anti-Cytokeratin, Acidic (Type I or LMW) Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | KRT77 |
|---|---|
| Synonyms | KRT1B |
| Tissue Location | Expressed exclusively in skin. |
For Research Use Only. Not For Use In Diagnostic Procedures.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
This MAb recognizes the 56.5kDa (CK10); 50kDa (CK14); 50kDa (CK15); 48kDa (CK16); 40kDa (CK19) keratins of the acidic (Type I or LMW) subfamily. Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50 , 48, 46, 45, and 40kDa. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.
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