癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
MKL1 antibody - C-terminal region
Rabbit Polyclonal Antibody
- 产品详情
- 实验流程
Application 
| WB |
|---|---|
| Primary Accession | Q8K4J6 |
| Other Accession | NM_153049, NP_694629 |
| Reactivity | Human, Mouse, Rat, Rabbit, Zebrafish, Pig, Dog, Horse, Bovine |
| Predicted | Human, Mouse, Rat, Rabbit, Pig, Chicken, Dog, Horse, Bovine |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | 102546 Da |
| Gene ID | 223701 |
|---|---|
| Alias Symbol | Mal, AMKL, Bsac, Mrtf-A, AI852829, AW743281, AW821984 |
| Other Names | MKL/myocardin-like protein 1, Basic SAP coiled-coil transcription activator, Megakaryoblastic leukemia 1 protein homolog, Myocardin-related transcription factor A, MRTF-A, Mkl1, Bsac |
| Format | Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Reconstitution & Storage | Add 100 ul of distilled water. Final anti-MKL1 antibody concentration is 1 mg/ml in PBS buffer with 2% sucrose. For longer periods of storage, store at 20°C. Avoid repeat freeze-thaw cycles. |
| Precautions | MKL1 antibody - C-terminal region is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | Mrtfa |
|---|---|
| Function | Transcription coactivator that associates with the serum response factor (SRF) transcription factor to control expression of genes regulating the cytoskeleton during development, morphogenesis and cell migration (PubMed:12019265, PubMed:12732141, PubMed:17588931, PubMed:19350017, PubMed:24732378). The SRF-MRTFA complex activity responds to Rho GTPase-induced changes in cellular globular actin (G- actin) concentration, thereby coupling cytoskeletal gene expression to cytoskeletal dynamics (PubMed:24732378). MRTFA binds G-actin via its RPEL repeats, regulating activity of the MRTFA-SRF complex (PubMed:12732141, PubMed:17588931). Activity is also regulated by filamentous actin (F-actin) in the nucleus (PubMed:23558171, PubMed:25759381). |
| Cellular Location | Cytoplasm. Nucleus. Note=Subcellular location is tightly regulated by actin both in cytoplasm and nucleus: high levels of G- actin in the nucleus observed during serum deprivation lead to low levels of nuclear MRTFA, while reduced levels of nuclear G-actin result in accumulation of MRTFA in the nucleus (PubMed:17588931, PubMed:21673315). G-actin-binding in the cytoplasm inhibits nuclear import by masking the nuclear localization signal (NLS) (PubMed:17588931, PubMed:20818336, PubMed:21673315). In contrast, binding to nuclear globular actin (G-actin) promotes nuclear export to the cytoplasm (PubMed:17588931). Nuclear localization is regulated by MICAL2, which mediates depolymerization of nuclear actin, which decreases nuclear G-actin pool, thereby promoting retention of MRTFA in the nucleus and subsequent formation of an active complex with SRF (By similarity). Nuclear import is mediated by importins KPNA4 and KPNB1 (PubMed:20818336). {ECO:0000250|UniProtKB:Q969V6, ECO:0000269|PubMed:17588931, ECO:0000269|PubMed:20818336, ECO:0000269|PubMed:21673315} |
| Tissue Location | Expressed in heart, brain, spleen, lung, liver, muscle, kidney and testis. |
Research Areas
For Research Use Only. Not For Use In Diagnostic Procedures.
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
REFERENCES
Sasazuki,T., et al., (2002) J. Biol. Chem. 277 (32), 28853-28860Reconstitution and Storage:For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
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