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GAPDH antibody - N-terminal region

Rabbit Polyclonal Antibody

     
  • 1 - GAPDH antibody - N-terminal region AI11693

    Host: Rabbit
    Target Name: GAPDH
    Sample Tissue: Hela Whole cell lysate
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    Antibody Dilution: 1.0μg/mlGAPDH is strongly supported by BioGPS gene expression data to be expressed in Human HeLa cells
  • 1 - GAPDH antibody - N-terminal region AI11693

    WB Suggested Anti-GAPDH antibody Titration: 1 μg/ml
    Sample Type: Human Raji
  • 1 - GAPDH antibody - N-terminal region AI11693

    WB Suggested Anti-GAPDH Antibody Titration: 0.2-1 μg/ml
    ELISA Titer: 1:62500
    Positive Control: Human brain
  • 1 - GAPDH antibody - N-terminal region AI11693
    Lanes: 1. 50 ug NIH3T3 cytosolic extract 2. 50 ug NIH3T3 cytosolic extract 3. 50 ug NIH3T3 cytosolic extract 4. 50 ug NIH3T3 cytosolic extract
    Primary Antibody Dilution: 1:1000
    Secondary Antibody: Anti-Rabbit HRP
    Secondary Antibody Dilution: 1:30000
    Gene Name: GAPDH
    Submitted by: Dr. Arimdam Basu, Dept. of Animal Biology, University of Pennsylvania
  • 1 - GAPDH antibody - N-terminal region AI11693

    Host: Rabbit
    Target Name:GAPDH
    Sample Tissue:293T
    Lane A: Primary Antibody
    Lane B: Primary Antibody + Blocking Peptide
    Primary Antibody
    Concentration:1μg/ml
    Peptide Concentration: 5μg/ml
    Lysate Quantity: 25ug/lane/lane Gel
    Concentration: 0.12
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB
Primary Accession Q2TSD0
Other Accession NM_002046, NP_002037
Reactivity Human, Mouse, Rat, Rabbit, Goat, Dog, Horse, Bovine, Sheep
Predicted Human, Mouse, Rat, Rabbit, Pig, Bovine, Sheep
Host Rabbit
Clonality Polyclonal
Calculated MW 36 KDa
Additional Information
Alias Symbol G3PD, GAPD, MGC88685
Format Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reconstitution & Storage Add 50 ul of distilled water. Final anti-GAPDH antibody concentration is 1 mg/ml in PBS buffer with 2% sucrose. For longer periods of storage, store at 20°C. Avoid repeat freeze-thaw cycles.
PrecautionsGAPDH antibody - N-terminal region is for research use only and not for use in diagnostic or therapeutic procedures.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Research Areas

REFERENCES

Rinne,T., (2008) Hum. Mol. Genet. 17 (13), 1968-1977 Reconstitution and Storage:For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.Publications:Fu, Y. et al. ABCA12 Regulates ABCA1-Dependent Cholesterol Efflux from Macrophages and the Development of Atherosclerosis. Cell Metab. 18, 225-38 (2013). WB, IP, ICC/IF, Sheep, Human, Guinea pig, Dog, H, Rabbit, Rat, Goat, Mouse, Bovine23931754Kalinec, G. M. et al. Acetaminophen and NAPQI are toxic to auditory cells via oxidative and endoplasmic reticulum stress-dependent pathways. Hear. Res. 313, 26-37 (2014). WB, Sheep, Human, Guinea pig, Dog, H, Rabbit, Rat, Goat, Mouse, Bovine24793116

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