SYMPK antibody - N-terminal region

Rabbit Polyclonal Antibody

Sample Type : Human HT29 cells
Primary Antibody Dilution : 1:50
Secondary Antibody : Donkey anti Rabbit-Alexa Fluor 488 Secondary Antibody Dilution : 1:500 Color/Signal Descriptions : Green: Symplekin Blue: DAPI
Gene Name : SYMPK Submitted by : Louise Lagerqvist (PhD), Oncology Department, Institute of Functional Genomics, Montpellier, France.
WB Suggested Anti-SYMPK Antibody Titration: 0.2-1 μg/ml
ELISA Titer: 1:1562500
Positive Control: Human Liver

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC
Primary Accession	Q92797
Other Accession	NM_004819, NP_004810
Reactivity	Human, Mouse, Rat, Rabbit, Pig, Dog, Guinea Pig, Horse, Bovine
Predicted	Human, Mouse, Rabbit, Pig, Dog, Guinea Pig, Horse, Bovine
Host	Rabbit
Clonality	Polyclonal
Calculated MW	141148 Da

Additional Information
Gene ID	8189
Alias Symbol	FLJ27092, SPK, SYM
Other Names	Symplekin, SYMPK, SPK
Format	Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reconstitution & Storage	Add 50 ul of distilled water. Final anti-SYMPK antibody concentration is 1 mg/ml in PBS buffer with 2% sucrose. For longer periods of storage, store at 20°C. Avoid repeat freeze-thaw cycles.
Precautions	SYMPK antibody - N-terminal region is for research use only and not for use in diagnostic or therapeutic procedures.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	SYMPK
Synonyms	SPK
Function	Scaffold protein that functions as a component of a multimolecular complex involved in histone mRNA 3'-end processing. Specific component of the tight junction (TJ) plaque, but might not be an exclusively junctional component. May have a house-keeping rule. Is involved in pre-mRNA polyadenylation. Enhances SSU72 phosphatase activity.
Cellular Location	Cytoplasm, cytoskeleton. Cell junction, tight junction. Cell membrane; Peripheral membrane protein; Cytoplasmic side Cell junction. Nucleus, nucleoplasm. Note=Cytoplasmic face of adhesion plaques (major) and nucleoplasm (minor) (in cells with TJ). Nucleoplasm (in cells without TJ). Nuclear bodies of heat-stressed cells Colocalizes with HSF1 in nuclear stress bodies upon heat shock (PubMed:14707147).
Tissue Location	In testis, expressed in polar epithelia and Sertoli cells but not in vascular endothelia. The protein is detected in stomach, duodenum, pancreas, liver, fetal brain, carcinomas, lens- forming cells, fibroblasts, lymphocytes, lymphoma cells, erythroleukemia cells but not in endothelium of vessels, epidermis, intercalated disks, Purkinje fiber cells of the heart and lymph node

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.