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HERC5 antibody - N-terminal region
Rabbit Polyclonal Antibody
- 产品详情
- 实验流程
Application 
| WB |
|---|---|
| Primary Accession | Q9UII4 |
| Other Accession | NM_016323, NP_057407 |
| Reactivity | Human, Dog, Bovine |
| Predicted | Human, Dog, Bovine |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | 116852 Da |
| Gene ID | 51191 |
|---|---|
| Alias Symbol | CEB1, CEBP1 |
| Other Names | E3 ISG15--protein ligase HERC5, 6.3.2.-, Cyclin-E-binding protein 1, HECT domain and RCC1-like domain-containing protein 5, HERC5, CEB1, CEBP1 |
| Format | Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Reconstitution & Storage | Add 50 ul of distilled water. Final anti-HERC5 antibody concentration is 1 mg/ml in PBS buffer with 2% sucrose. For longer periods of storage, store at 20°C. Avoid repeat freeze-thaw cycles. |
| Precautions | HERC5 antibody - N-terminal region is for research use only and not for use in diagnostic or therapeutic procedures. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | HERC5 {ECO:0000303|PubMed:38421872, ECO:0000312|HGNC:HGNC:24368} |
|---|---|
| Function | Major E3 ligase for ISG15 conjugation (PubMed:16407192, PubMed:16815975, PubMed:16884686, PubMed:20308324, PubMed:20542004, PubMed:26355087, PubMed:27534820, PubMed:27564865, PubMed:37279284, PubMed:38421872, PubMed:38652662, PubMed:40184173). Functions as part of the ISGylation machinery that recognizes target proteins in a broad and relatively non-specific manner (PubMed:16407192, PubMed:16815975, PubMed:16884686, PubMed:20308324, PubMed:20542004, PubMed:26355087, PubMed:27534820, PubMed:27564865, PubMed:37279284). Physically associated with polyribosomes, broadly modifies newly synthesized proteins in a cotranslational manner (PubMed:20542004). Acts as a positive regulator of innate antiviral response in cells induced by interferon by mediating ISGylation of proteins involved in innate immunity, such as CGAS, IRF3, IFIH1/MDA5 and STING1 (PubMed:16815975, PubMed:20308324, PubMed:38421872, PubMed:38652662, PubMed:40184173). Catalyzes ISGylation of IRF3 which results in sustained activation, it attenuates IRF3-PIN1 interaction, which antagonizes IRF3 ubiquitination and degradation, and boosts the antiviral response (PubMed:20308324). Also regulates innate antiviral response by mediating ISGylation of CGAS and STING1, thereby promoting activation of cGAS-STING signaling (PubMed:38421872, PubMed:38652662). Catalyzes ISGylation of IFIH1/MDA5, promoting IFIH1/MDA5 oligomerization and immune signal transduction (PubMed:40184173). Also regulates innate immunity by catalyzing ISGylation of viral proteins: newly translated viral proteins are primary targets of ISG15 in interferon-stimulated cells (PubMed:20133869, PubMed:20385878, PubMed:20542004, PubMed:22093708, PubMed:34572049). Interferes with the function of key viral structural proteins such as ebolavirus structural protein VP40 or HIV-1 protein GAG (PubMed:22093708, PubMed:34572049). Catalyzes ISGylation of influenza A viral NS1 which attenuates virulence; ISGylated NS1 fails to form homodimers and thus to interact with its RNA targets (PubMed:20133869, PubMed:20385878). Catalyzes ISGylation of papillomavirus type 16 L1 protein which results in dominant-negative effect on virus infectivity (PubMed:20542004). Mediates ISGylation of the phosphatase PTEN leading to its degradation, thus alleviating its suppression of the PI3K-AKT signaling pathway and promoting the production of cytokines that facilitate bacterial clearance (PubMed:37279284). Promotes parkin/PRKN ubiquitin E3 ligase activity by suppressing the intramolecular interaction that maintains its autoinhibited conformation (PubMed:27534820). |
| Cellular Location | Cytoplasm, perinuclear region. Cytoplasm. Note=Associated with the polyribosomes, probably via the 60S subunit. |
| Tissue Location | Expressed in testis and to a lesser degree in brain, ovary and placenta. Found in most tissues at low levels |
Research Areas
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
REFERENCES
Salon,C.,(2007)Oncogene26(48),6927-6936ReconstitutionandStorage:Forshorttermuse,storeat2-8Cupto1week.Forlongtermstorage,storeat-20Cinsmallaliquotstopreventfreeze-thawcycles.
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