PDIA6 antibody - N-terminal region

Rabbit Polyclonal Antibody

Sample Type:Â NT2 cells Red:Â Antibody Blue:Â DAPI Primary Dilution:Â 1ug/50ul antibody
Secondary Antibody:Â Alexa goat anti-rabbit 594 Image
Submitted by:Â Yuzhi Chen, University of Arkansas for Medical Sciences Â
Sample Type :Mouse Brain lysate
Amount and Sample Type : 500 ug Human NT2 cell lysate
Amount of IP Antibody : 6 ug Primary Antibody : PDIA6
Primary Antibody Dilution : 1:500
Secondary Antibody : Goat anti-rabbit Alexa-Fluor 594 Secondary Antibody Dilution : 1:5000
Gene Name : PDIA6
WB Suggested Anti-PDIA6 Antibody Titration: 0.2-1 μg/ml
ELISA Titer: 1:62500
Positive Control: 721_B cell lysate
PDIA6 is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, IP
Primary Accession	Q922R8
Other Accession	NM_005742, NP_005733
Reactivity	Human, Mouse, Rat, Rabbit, Pig, Dog, Horse, Bovine
Predicted	Human, Mouse, Rat, Pig, Chicken, Dog, Horse, Bovine
Host	Rabbit
Clonality	Polyclonal
Calculated MW	48100 Da

Additional Information
Gene ID	71853
Alias Symbol	ERP5, P5, TXNDC7
Other Names	Protein disulfide-isomerase A6, 5.3.4.1, Thioredoxin domain-containing protein 7, Pdia6, Txndc7
Format	Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reconstitution & Storage	Add 50 ul of distilled water. Final anti-PDIA6 antibody concentration is 1 mg/ml in PBS buffer with 2% sucrose. For longer periods of storage, store at 20°C. Avoid repeat freeze-thaw cycles.
Precautions	PDIA6 antibody - N-terminal region is for research use only and not for use in diagnostic or therapeutic procedures.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	Pdia6
Synonyms	Txndc7
Function	May function as a chaperone that inhibits aggregation of misfolded proteins (PubMed:24508390). Negatively regulates the unfolded protein response (UPR) through binding to UPR sensors such as ERN1, which in turn inactivates ERN1 signaling (By similarity). May also regulate the UPR via the EIF2AK3 UPR sensor (By similarity). Plays a role in platelet aggregation and activation by agonists such as convulxin, collagen and thrombin (By similarity).
Cellular Location	Endoplasmic reticulum lumen {ECO:0000250\|UniProtKB:Q15084}. Cell membrane {ECO:0000250\|UniProtKB:Q15084}. Melanosome {ECO:0000250\|UniProtKB:Q15084}

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.