癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
Ska1 antibody - middle region
Rabbit Polyclonal Antibody
- 产品详情
- 实验流程
Application 
| WB |
|---|---|
| Primary Accession | Q9CPV1 |
| Other Accession | NM_025581, NP_079857 |
| Reactivity | Human, Mouse, Rat, Rabbit, Pig, Dog, Horse, Bovine |
| Predicted | Human, Mouse, Rat, Rabbit, Dog, Bovine |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | 29459 Da |
| Gene ID | 66468 |
|---|---|
| Alias Symbol | 2810433K01Rik, AV117428 |
| Other Names | Spindle and kinetochore-associated protein 1, Ska1 |
| Format | Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Reconstitution & Storage | Add 50 ul of distilled water. Final anti-Ska1 antibody concentration is 1 mg/ml in PBS buffer with 2% sucrose. For longer periods of storage, store at 20°C. Avoid repeat freeze-thaw cycles. |
| Precautions | Ska1 antibody - middle region is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | Ska1 |
|---|---|
| Function | Component of the SKA complex, a microtubule plus end-binding complex of the outer kinetochore that stabilizes spindle microtubule- kinetochore attachments, promotes alignment of chromosomes at the mitotic spindle equator (chromosome congression) and assists suppression of the spindle assembly checkpoint (By similarity). Kinetochores, consisting of a centromere-associated inner segment and a microtubule-contacting outer segment, play a crucial role in chromosome segregation by mediating the physical connection between centromeric DNA and spindle microtubules (By similarity). The outer kinetochore is made up of the ten-subunit KMN network complex, comprising the MIS12, NDC80 and KNL1 complexes, and auxiliary microtubule-associated components such as the SKA complex; together they connect the outer kinetochore with the inner kinetochore, bind microtubules, and mediate interactions with mitotic checkpoint proteins that delay anaphase until chromosomes are bioriented on the spindle (By similarity). The SKA complex is loaded onto bioriented kinetochores and it facilitates chromosome congression by stabilizing microtubules together with MAPRE1, and end-on attachment of the NDC80 complex to depolymerizing spindle microtubules, thereby assisting the poleward-moving kinetochore in withstanding microtubule pulling forces (By similarity). The complex associates with dynamic microtubule plus-ends and can track both depolymerizing and elongating microtubules (By similarity). The complex recruits protein phosphatase 1 (PP1) to the kinetochore in prometaphase and metaphase, to oppose spindle assembly checkpoint signaling and promote the onset of anaphase (By similarity). In the complex, it mediates interactions with microtubules (By similarity). It also stimulates AURKB/Aurora B catalytic activity (By similarity). During meiosis the SKA complex stabilizes the meiotic spindle and is required for its migration to the cortex (PubMed:22336914). |
| Cellular Location | Cytoplasm, cytoskeleton, spindle. Chromosome, centromere, kinetochore {ECO:0000250|UniProtKB:Q96BD8}. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome {ECO:0000250|UniProtKB:Q96BD8} Note=Localizes to bioriented kinetochores and spindle microtubules during metaphase in a NDC80 complex-dependent manner (By similarity) The SKA complex begins to concentrate at kinetochores before microtubule attachment but reaches maximum levels on bioriented metaphase chromosomes (By similarity). Localizes both to microtubule plus-ends and along the length of microtubules (By similarity). The localization of the SKA complex to kinetochores is positively regulated by kinase CDK1 (By similarity). The localization of the SKA complex to kinetochores is negatively regulated by protein serine/threonine kinase AURKB, and this action is opposed directly or indirectly by the PP1 and PP2A protein phosphatase complexes (By similarity). Localizes at the centrosome during interphase and prophase (By similarity). Localizes to the meiotic spindle, but not to kinetochores, from the stage of germinal vesicle breakdown (GVBD) to meiosis II (MII) (PubMed:22336914). {ECO:0000250|UniProtKB:Q96BD8, ECO:0000269|PubMed:22336914} |
Research Areas
For Research Use Only. Not For Use In Diagnostic Procedures.
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
REFERENCES
Chatterjee B.,et al.Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases.
Carninci P.,et al.Science 309:1559-1563(2005).
终于等到您。ABCEPTA（百远生物）抗体产品。
点击下方“我要评价 ”按钮提交您的反馈信息，您的反馈和评价是我们最宝贵的财富之一,
我们将在1-3个工作日内处理您的反馈信息。
如有疑问，联系：0512-88856768 tech-china@abcepta.com.
