Sema3f antibody - N-terminal region

Rabbit Polyclonal Antibody

Sample Type : Untransfected HEK293 and Sema3F-AP transfected HEK293
Primary Antibody Dilution : 1:1000
Secondary Antibody : Anti rabbit-Alexa Fluor 488 Secondary Antibody Dilution : 1:500 Color/Signal Descriptions :
Gene Name : SEMA3F Submitted by : Dr. Tracy Tran, Rutgers University
Researcher: Dr. Chrisna LeVaillant, School of Anatomy and Human Biology
Application: IP
Species+tissue/cell type:1.5mL conditioned media from HEK293F cells transfected with 1: Sema 3F_AP, 2: Sema3C_FLAG, 3: UntransfectedAmount of IP Antibody: Amount of IP Antibody: 5ug
Primary Antibody Dilution: 1:1000
Secondary Antibody: Goat Anti-rabbit HRP
Secondary Antibody Dilution:1:10000
WB Suggested Anti-Sema3f Antibody Titration: 1.0 μg/ml
Positive Control: Mouse Liver

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, IP
Primary Accession	O88632-2
Other Accession	NM_011349, NP_035479
Reactivity	Human, Mouse, Rat, Rabbit, Zebrafish, Pig, Dog, Guinea Pig, Horse, Bovine
Predicted	Human, Mouse, Rat, Rabbit, Zebrafish, Pig, Chicken, Dog, Horse, Bovine
Host	Rabbit
Clonality	Polyclonal
Calculated MW	83 KDa

Additional Information
Alias Symbol	Sema4, Semak
Format	Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reconstitution & Storage	Add 50 ul of distilled water. Final anti-Sema3f antibody concentration is 1 mg/ml in PBS buffer with 2% sucrose. For longer periods of storage, store at 20°C. Avoid repeat freeze-thaw cycles.
Precautions	Sema3f antibody - N-terminal region is for research use only and not for use in diagnostic or therapeutic procedures.

For Research Use Only. Not For Use In Diagnostic Procedures.