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GPR65 antibody - C-terminal region
Rabbit Polyclonal Antibody
- 产品详情
- 实验流程
Application 
| WB |
|---|---|
| Primary Accession | Q8IYL9 |
| Other Accession | NM_003608, NP_003599 |
| Reactivity | Human, Mouse, Rat, Rabbit, Pig, Dog, Horse |
| Predicted | Human, Mouse, Rat, Rabbit, Pig, Dog, Horse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | 39333 Da |
| Gene ID | 8477 |
|---|---|
| Alias Symbol | TDAG8, hTDAG8 |
| Other Names | Psychosine receptor, G-protein coupled receptor 65, T-cell death-associated gene 8 protein, GPR65, TDAG8 |
| Format | Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Reconstitution & Storage | Add 50 ul of distilled water. Final anti-GPR65 antibody concentration is 1 mg/ml in PBS buffer with 2% sucrose. For longer periods of storage, store at 20°C. Avoid repeat freeze-thaw cycles. |
| Precautions | GPR65 antibody - C-terminal region is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | GPR65 {ECO:0000303|PubMed:27287411, ECO:0000312|HGNC:HGNC:4517} |
|---|---|
| Function | Proton-sensing G-protein coupled receptor activated by extracellular pH, which is required to monitor pH changes and generate adaptive reactions (PubMed:15326175, PubMed:15618224, PubMed:20855608, PubMed:33478938, PubMed:37722051, PubMed:39753132). Activated by an optimal pH of 7.4 (PubMed:39753132). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide- binding proteins (G proteins) and modulates the activity of downstream effectors, such as adenylate cyclase (PubMed:15326175, PubMed:15618224, PubMed:37722051, PubMed:39753132). GPR65 is mainly coupled to G(s) G proteins and mediates activation of adenylate cyclase activity (PubMed:15618224, PubMed:37722051, PubMed:39753132). May also act as a receptor for the glycosphingolipid psychosine (PSY) and several related glycosphingolipids (PubMed:11309421, PubMed:15326175). Plays a role in immune response by maintaining lysosome function and regulating T-cell metabolism (PubMed:27287411). Acts as a regulator of inflammation by mediating pH-sensing of extracellular acidification which takes place in inflamed tissues: activation regulates endo-lysosomal function of immune cells and T-cell metabolism (By similarity). Constitutively active in endosomes and stimulates adenylate cyclase production from endosomes independently from extracellular pH changes (PubMed:39753132). |
| Cellular Location | Cell membrane; Multi-pass membrane protein. Early endosome membrane; Multi-pass membrane protein. Late endosome membrane; Multi-pass membrane protein. Note=Internalizes and localizes to early and late endosomes, from where GPR65 signals at steady state, irrespective of extracellular pH (PubMed:39753132). Changes in extracellular pH may relocalize receptor signaling to the cell membrane (PubMed:39753132). |
| Tissue Location | Predominantly expressed in thymus, spleen, lymph nodes, small intestine, lung, placenta and peripheral blood leukocytes |
Research Areas
For Research Use Only. Not For Use In Diagnostic Procedures.
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
REFERENCES
Kyaw H.,et al.DNA Cell Biol. 17:493-500(1998).
Heilig R.,et al.Nature 421:601-607(2003).
Im D.-S.,et al.J. Cell Biol. 153:429-434(2001).
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