Anti-MAF1 Antibody (Internal)

Rabbit Anti Human Polyclonal Antibody

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P
Primary Accession	Q9H063
Predicted	Human, Mouse, Rat, Bovine
Host	Rabbit
Clonality	Polyclonal
Calculated MW	28771 Da
Concentration (mg/ml)	1 mg/ml

Additional Information
Gene ID	84232
Alias Symbol	MAF1
Other Names	MAF1, Homolog of yeast MAF1, MAF1 homolog (S. cerevisiae)
Target/Specificity	Recognizes endogenous levels of MAF1 protein.
Reconstitution & Storage	Immunoaffinity purified
Precautions	Anti-MAF1 Antibody (Internal) is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	MAF1
Function	Plays a role in the repression of RNA polymerase III-mediated transcription in response to changing nutritional, environmental and cellular stress conditions to balance the production of highly abundant tRNAs, 5S rRNA, and other small non-coding RNAs with cell growth and maintenance (PubMed:18377933, PubMed:20233713, PubMed:20516213, PubMed:20543138). Also plays a key role in cell fate determination by promoting mesorderm induction and adipocyte differentiation (By similarity). Mechanistically, associates with the RNA polymerase III clamp and thereby impairs its recruitment to the complex made of the promoter DNA, TBP and the initiation factor TFIIIB (PubMed:17505538, PubMed:20887893). When nutrients are available and mTOR kinase is active, MAF1 is hyperphosphorylated and RNA polymerase III is engaged in transcription. Stress-induced MAF1 dephosphorylation results in nuclear localization, increased targeting of gene-bound RNA polymerase III and a decrease in the transcriptional readout (PubMed:26941251). Additionally, may also regulate RNA polymerase I and RNA polymerase II- dependent transcription through its ability to regulate expression of the central initiation factor TBP (PubMed:17499043).
Cellular Location	Nucleus. Cytoplasm