HIS Tag Antibody
Mouse Monoclonal Antibody (Mab)
- 产品详情
- 文献引用 : 50
- 实验流程
- 背景知识
Application ![]()
| WB, E |
---|---|
Reactivity | Human |
Host | Mouse |
Clonality | Monoclonal |
Isotype | Mouse IgG1 |
Clone Names | 6AT18 |
Target/Specificity | Purified recombinant HIS-tagged fusion protein and poly-HIS peptide were used to produced this monoclonal antibody. |
---|---|
Dilution | WB~~1:4000 |
Format | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | HIS Tag Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
For Research Use Only. Not For Use In Diagnostic Procedures.

Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
Epitope tags consisting of short sequences recognized by well-characterized monoclonal antibodies have been widely used in the study of protein expression in various systems. The 6xHIS tag (HHHHHH), recognized by the monoclonal antibody clone 6AT18 provides an established example of this application. 6xHIS-tagged fusion proteins are easily purified from cell lysates by affinity chromatography using Nickel-Sepharose resin. Abgent's anti-6xHIS monoclonal antibody provides a simple solution to detect the expression of HIS-tagged fusion proteins in cells.
REFERENCES
Hochuli E, Doebeli H, and Schacher A. New metal chelate absorbent selective for proteins and peptides containing neighboring histidine residues. J. Chromatogr. 1987;411:177-184.

终于等到您。ABCEPTA(百远生物)抗体产品。
点击下方“我要评价 ”按钮提交您的反馈信息,您的反馈和评价是我们最宝贵的财富之一,
我们将在1-3个工作日内处理您的反馈信息。
如有疑问,联系:0512-88856768 tech-china@abcepta.com.