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Beta-actin Antibody

Purified Mouse Monoclonal Antibody (Mab)

     
  • 1 - Beta-actin Antibody AM1021B
    All lanes : Anti-Beta-actin Antibody at 1:2000 dilution Lane 1: A431 whole cell lysate Lane 2: MCF-7 whole cell lysate Lane 3: HepG2 whole cell lysate Lane 4: HL-60 whole cell lysate Lane 5: R. stomach whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Mouse IgG/A/M(H/L), Peroxidase conjugated at 1/2000 dilution. Observed band size : 42kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 14 - Beta-actin Antibody AM1021B
    Immunohistochemical analysis of paraffin-embedded H. spleen section using Beta-actin Antibody(Cat#AM1021b). AM1021b was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, E
Primary Accession P60709
Reactivity Human, Mouse, Rat
Host Mouse
Clonality Monoclonal
Isotype IgG
Clone Names 8H10D10
Calculated MW 41737 Da
Additional Information
Gene ID 60
Other Names Actin, cytoplasmic 1, Beta-actin, Actin, cytoplasmic 1, N-terminally processed, ACTB
Target/Specificity ACTB recombinant protein is used to produce this monoclonal antibody.
Dilution WB~~1:1000-1:2000
IHC-P~~1:100~500
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsBeta-actin Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ACTB
Function Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells (PubMed:29581253). Actin exists in both monomeric (G-actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction (PubMed:29581253). In addition to their role in the cytoplasmic cytoskeleton, G- and F-actin also localize in the nucleus, and regulate gene transcription and motility and repair of damaged DNA (PubMed:29925947).
Cellular Location Cytoplasm, cytoskeleton. Nucleus Note=Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
Research Areas

BACKGROUND

This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins.

REFERENCES

Sex-specific proteome differences in the anterior cingulate cortex of schizophrenia. Martins-de-Souza D, et al. J Psychiatr Res, 2010 Apr 8. PMID 20381070. Identification of a hormone-regulated dynamic nuclear actin network associated with estrogen receptor alpha in human breast cancer cell nuclei. Ambrosino C, et al. Mol Cell Proteomics, 2010 Jun. PMID 20308691. Contribution of rearranged actin structures to the spread of Ectromelia virus infection in vitro. Boratynska A, et al. Acta Virol, 2010. PMID 20201613. Molecular mechanisms underlying nucleocytoplasmic shuttling of actinin-4. Kumeta M, et al. J Cell Sci, 2010 Apr 1. PMID 20197409. Tyrosine phosphorylation of cofilin at Y68 by v-Src leads to its degradation through ubiquitin-proteasome pathway. Yoo Y, et al. Oncogene, 2010 Jan 14. PMID 19802004.

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