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ATG12 Antibody

Purified Mouse Monoclonal Antibody (Mab)

     
  • 1 - ATG12 Antibody AM1816a
    Anti-APG12 at 1:4000 dilution + ATG12 recombinant protein Lysates/proteins at 20ng per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 26 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, E
Primary Accession O94817
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG2b, Igk
Clone Names 43CT73.3.5.5.4
Calculated MW 15113 Da
Additional Information
Gene ID 9140
Other Names Ubiquitin-like protein ATG12, Autophagy-related protein 12, APG12-like, ATG12, APG12, APG12L
Target/Specificity APG12L recombinant protein is used to produce this monoclonal antibody.
Dilution WB~~1:4000
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsATG12 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ATG12
Synonyms APG12, APG12L
Function Ubiquitin-like protein involved in autophagy vesicles formation. Conjugation with ATG5 through a ubiquitin-like conjugating system involving also ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes.
Cellular Location Cytoplasm. Preautophagosomal structure membrane; Peripheral membrane protein. Note=TECPR1 recruits the ATG12- ATG5 conjugate to the autolysosomal membrane
Tissue Location Ubiquitous..
Research Areas

BACKGROUND

Autophagy is a process of bulk protein degradation in which cytoplasmic components, including organelles, are enclosed in double-membrane structures called autophagosomes and delivered to lysosomes or vacuoles for degradation. ATG12 is the human homolog of a yeast protein involved in autophagy (Mizushima et al., 1998 [PubMed 9852036]).

REFERENCES

Control of basal autophagy by calpain1 mediated cleavage of ATG5. Xia HG, et al. Autophagy, 2010 Jan. PMID 19901552.
Frameshift mutations of autophagy-related genes ATG2B, ATG5, ATG9B and ATG12 in gastric and colorectal cancers with microsatellite instability. Kang MR, et al. J Pathol, 2009 Apr. PMID 19197948.
Regulation of autophagy by the p300 acetyltransferase. Lee IH, et al. J Biol Chem, 2009 Mar 6. PMID 19124466.
Mitochondrial DNA deletions and chloramphenicol treatment stimulate the autophagic transcript ATG12. Prigione A, et al. Autophagy, 2007 Jul-Aug. PMID 17457038.
Large-scale mapping of human protein-protein interactions by mass spectrometry. Ewing RM, et al. Mol Syst Biol, 2007. PMID 17353931.

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