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Beclin 1 Antibody (Ascites) 精选

Mouse Monoclonal Antibody (Mab)

Fluorescent image of U251 cells stained with AM1818a Beclin1 antibody.U251 cells were treated with Chloroquine (50 μM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AM1818a Beclin1 primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-mouse antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). Beclin1 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.
Western blot analysis of anti-BECN1 Mab (Cat. #AM1818a) in HepG2, 293, Jurkat and Hela cell line lysates (35μg/lane). BECN1 (arrow) was detected using the Mab ascites (1:2000 dilution).
Western blot analysis of anti-BECN1 Mab (Cat. #AM1818a) in recombinant BECN1 protein. BECN1(arrow) was detected using the ascites Mab (1:2000 dilution).
Breast CA section stained with Autophagy Beclin 1 Antibody (Cat. # AM1818a) at a 1:50 dilution. Data courtesy of Dr. Anita Thyagarajan, Cancer Research Laboratory, Methodist Research Institute, Indianapolis, Indiana.

产品详情
文献引用 : 5
实验流程
背景知识

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	IF, WB, IHC-P, E
Primary Accession	Q14457
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Isotype	IgG3λ
Clone Names	15CT26
Calculated MW	51896 Da

Additional Information
Gene ID	8678
Other Names	Beclin-1, Coiled-coil myosin-like BCL2-interacting protein, Protein GT197, BECN1, GT197
Target/Specificity	This Beclin 1 antibody was raised using purified His-tagged recombinant full length human Autophagy BECN1.
Dilution	IF~~1:100 WB~~1:50~2000 IHC-P~~1:100~500 E~~Use at an assay dependent concentration.
Format	Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	Beclin 1 Antibody (Ascites) is for research use only and not for use in diagnostic or therapeutic procedures.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	BECN1
Synonyms	GT197
Function	Plays a central role in autophagy (PubMed:18570871, PubMed:21358617, PubMed:23184933, PubMed:23974797, PubMed:25484083, PubMed:28445460, PubMed:37776275). Acts as a core subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. Involved in regulation of degradative endocytic trafficking and required for the abscission step in cytokinesis, probably in the context of PI3KC3-C2 (PubMed:20208530, PubMed:20643123, PubMed:23974797, PubMed:26783301). Essential for the formation of PI3KC3-C2 but not PI3KC3-C1 PI3K complex forms. Involved in endocytosis (PubMed:25275521). May play a role in antiviral host defense.
Cellular Location	Cytoplasm. Golgi apparatus, trans-Golgi network membrane; Peripheral membrane protein. Endosome membrane; Peripheral membrane protein. Endoplasmic reticulum membrane; Peripheral membrane protein. Mitochondrion membrane; Peripheral membrane protein. Endosome {ECO:0000250\|UniProtKB:O88597} Cytoplasmic vesicle, autophagosome. Note=Interaction with ATG14 promotes translocation to autophagosomes. Expressed in dendrites and cell bodies of cerebellar Purkinje cells (By similarity) {ECO:0000250\|UniProtKB:O88597, ECO:0000269\|PubMed:19050071} [Beclin-1-C 37 kDa]: Mitochondrion {ECO:0000250\|UniProtKB:O88597}
Tissue Location	Ubiquitous.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

BACKGROUND

Beclin-1 participates in the regulation of autophagy and has an important role in development, tumorigenesis, and neurodegeneration (Zhong et al., 2009 [PubMed 19270693]).

REFERENCES

References for protein:
1.Age at onset in Huntington's disease is modified by the autophagy pathway: implication of the V471A polymorphism in Atg7. Metzger S, et al. Hum Genet, 2010 Oct. PMID 20697744.
2.Interaction of Beclin 1 with survivin regulates sensitivity of human glioma cells to TRAIL-induced apoptosis. Niu TK, et al. FEBS Lett, 2010 Aug 20. PMID 20638385.
3.Regulation of amyloid precursor protein processing by the Beclin 1 complex. Jaeger PA, et al. PLoS One, 2010 Jun 15. PMID 20559548.
4.Genetic and epigenetic silencing of the beclin 1 gene in sporadic breast tumors. Li Z, et al. BMC Cancer, 2010 Mar 16. PMID 20230646.
5.Over-expression of the Beclin1 gene upregulates chemosensitivity to anti-cancer drugs by enhancing therapy-induced apoptosis in cervix squamous carcinoma CaSki cells. Sun Y, et al. Cancer Lett, 2010 Aug 28. PMID 20207475.
References for U251 cell line:
1. Westermark B.; Pontén J.; Hugosson R. (1973).” Determinants for the establishment of permanent tissue culture lines from human gliomas”. Acta Pathol Microbiol Scand A. 81:791-805. [PMID: 4359449].
2. Pontén, J.,Westermark B. (1978).” Properties of Human Malignant Glioma Cells in Vitro”. Medical Biology 56: 184-193.[PMID: 359950].
3. Geng Y.;Kohli L.; Klocke B.J.; Roth K.A.(2010). “Chloroquine-induced autophagic vacuole accumulation and cell death in glioma cells is p53 independent”. Neuro Oncol. 12(5): 473–481.[ PMID: 20406898].

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¥ 1,250.00

Cat# AM1818a

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¥ 1,250.00

¥ 2,050.00

¥ 2,960.00

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Beclin 1 Antibody (Ascites) 精选

Mouse Monoclonal Antibody (Mab)

BACKGROUND

REFERENCES

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