癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
MGMT Antibody
Mouse Monoclonal Antibody (Mab)
- 产品详情
- 实验流程
- 背景知识
Application 
| WB, IHC-P, IF, E |
|---|---|
| Primary Accession | P16455 |
| Reactivity | Human |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone Names | 888CT22.2.4 |
| Calculated MW | 21646 Da |
| Gene ID | 4255 |
|---|---|
| Other Names | Methylated-DNA--protein-cysteine methyltransferase, 6-O-methylguanine-DNA methyltransferase, MGMT, O-6-methylguanine-DNA-alkyltransferase, MGMT |
| Target/Specificity | Purified His-tagged MGMT protein was used to produced this monoclonal antibody. |
| Dilution | WB~~1:1000 IHC-P~~1:100 IF~~1:25 E~~Use at an assay dependent concentration. |
| Format | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | MGMT Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | MGMT |
|---|---|
| Function | Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) and O4-methylthymine (O4-MeT) in DNA. Repairs the methylated nucleobase in DNA by stoichiometrically transferring the methyl group to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated. |
| Cellular Location | Nucleus. |
For Research Use Only. Not For Use In Diagnostic Procedures.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.
REFERENCES
Edara S., et al. Carcinogenesis 16:1637-1642(1995).
Tano K., et al. Proc. Natl. Acad. Sci. U.S.A. 87:686-690(1990).
Rydberg B., et al. J. Biol. Chem. 265:9563-9569(1990).
Koike G., et al. J. Biol. Chem. 265:14754-14762(1990).
Hayakawa H., et al. J. Mol. Biol. 213:739-747(1990).
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