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CD73(NT5E) Antibody (C-term)

Mouse Monoclonal Antibody (Mab)

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AM2223A staining CD73 (NT5E) in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
AM2223A staining CD73 (NT5E) in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
Anti-CD73 (NT5E) Antibody (C-term) at 1:4000 dilution + MDA-MB-231 whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 63 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Anti-CD73 (NT5E) Antibody (C-term) at 1:2000 dilution + WiDr whole cell lysates Lysates/proteins at 20 μg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 63 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

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背景知识

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, E
Primary Accession	P21589
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Isotype	IgG1
Clone Names	974CT7.5.3
Calculated MW	63368 Da

Additional Information
Gene ID	4907
Other Names	5'-nucleotidase, 5'-NT, Ecto-5'-nucleotidase, CD73, NT5E, NT5, NTE
Target/Specificity	Purified His-tagged CD73(NT5E) protein was used to produced this monoclonal antibody.
Dilution	WB~~1:2000 IHC-P~~1:100~500 E~~Use at an assay dependent concentration.
Format	Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	CD73(NT5E) Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	NT5E
Synonyms	NT5, NTE
Function	Catalyzes the hydrolysis of nucleotide monophosphates, releasing inorganic phosphate and the corresponding nucleoside, with AMP being the preferred substrate (PubMed:21933152, PubMed:22997138, PubMed:23142347, PubMed:24887587, PubMed:34403084). Shows a preference for ribonucleotide monophosphates over their equivalent deoxyribose forms (PubMed:34403084). Other substrates include IMP, UMP, GMP, CMP, dAMP, dCMP, dTMP, NAD and NMN (PubMed:21933152, PubMed:22997138, PubMed:23142347, PubMed:24887587, PubMed:34403084).
Cellular Location	Cell membrane; Lipid-anchor, GPI-anchor

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

BACKGROUND

Hydrolyzes extracellular nucleotides into membrane permeable nucleosides.

REFERENCES

Misumi Y., et al. Eur. J. Biochem. 191:563-569(1990).
Otsuki T., et al. DNA Res. 12:117-126(2005).
Mungall A.J., et al. Nature 425:805-811(2003).
Hansen K.R., et al. Gene 167:307-312(1995).
Zanoni L., et al. Submitted (MAY-1998) to the EMBL/GenBank/DDBJ databases.

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¥ 1,250.00

Cat# AM2223a

Size:

50 µl

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Price:

¥ 1,250.00

¥ 2,050.00

¥ 2,960.00

¥ 4,550.00

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CD73(NT5E) Antibody (C-term)

Mouse Monoclonal Antibody (Mab)

BACKGROUND

REFERENCES

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