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PPP2R1B Antibody

Purified Mouse Monoclonal Antibody (Mab)

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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U-2 OS (human bone osteosarcoma cell line) cells labeling PPP2R1B with AM8469b at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-mouse IgG (NA166821) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on U-2 OS cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin (PD18466410) at 1/100 dilution (red).The nuclear counter stain is DAPI (blue).
AM8469b staining PPP2R1B in human spleen sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
Overlay histogram showing Jurkat cells stained with AM8469b (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AM8469b, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(NA168821)) at 1/400 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was mouse IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
All lanes : Anti-PPP2R1B Antibody at 1:4000 dilution Lane 1: Jurkat whole cell lysates Lane 2: human brain lysates Lane 3: mouse brain lysates Lane 4: mouse lung lysates Lane 5: rat brain lysates Lysates/proteins at 20 μg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 66 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, FC, IHC-P, IF, E
Primary Accession	P30154
Reactivity	Human, Rat, Mouse
Host	Mouse
Clonality	monoclonal
Isotype	IgG1,Κ
Clone Names	1496CT356.164.25.226
Calculated MW	66214 Da

Additional Information
Gene ID	5519
Other Names	Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A beta isoform, PP2A subunit A isoform PR65-beta, PP2A subunit A isoform R1-beta, PPP2R1B
Target/Specificity	This PPP2R1B antibody is generated from a mouse immunized with a recombinant protein of human PPP2R1B.
Dilution	WB~~1:4000 FC~~1:25 IHC-P~~1:100~500 IF~~1:25 E~~Use at an assay dependent concentration.
Format	Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	PPP2R1B Antibody is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	PPP2R1B
Function	The PR65 subunit of protein phosphatase 2A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit.

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

BACKGROUND

The PR65 subunit of protein phosphatase 2A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit.

REFERENCES

Baysal B.E.,et al.Gene 217:107-116(1998).
Wang S.S.,et al.Science 282:284-287(1998).
Baysal B.E.,et al.Eur. J. Hum. Genet. 9:121-129(2001).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Taylor T.D.,et al.Nature 440:497-500(2006).

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¥ 1,250.00

Cat# AM8469b

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PPP2R1B Antibody

Purified Mouse Monoclonal Antibody (Mab)

BACKGROUND

REFERENCES

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